Chromic-P32 phosphate treatment of implanted pancreatic carcinoma: Mechanism involved

Table 1 Fourteen day-absorbed doses in tumor, tumor weight, tumor cell necrosis rate, MVD, PI and percentage of loosely arranged tumor cells to remaining tumor cells (n = 6).

Group	Injected dose(MBq)	Absorbed dose (Gy)	14 d tumorweight (g)	Tumor cell necroticrate(%)	F	SNK1	MVD2	PI
6	3.7	248.7	2.16±0.15	20	4.14	A	38.5±4.0	70
7	7.4	497.4	1.60±0.12	45	33.57	B	28.0±2.9	65
8	14.8	852.7	1.13±0.10	65	44.62	C	17.0±2.9	45
9	18.5	1147.8	0.86±0.09	70	54.93	C	8.8±1.5	20
10	29.6	2023.4	0.30±0.05	95	105.36	D	5.7±2.3	10
11	0	–	2.84±0.17	4	–	E	65.0±5.2	92

¹Variance analysis after conversion of tumor cell necrosis rate to square root in each group compared with 0 MBq group, F = 4.14-105.36, P<0.01, SNK test for cell necrosis rate: Same letter means no statistical difference of mean values among groups; differed letters mean the statistical difference among groups.

²Comparison of MVD between medication and control groups, t = 11.9-26.1, P<0.01, all with prominent differences.

Table 2 Absorbed dose, rates of Pc-3 cell apoptosis and necrosis, percentages of Apo2.7 and caspase-3 positive expression (%) and Bcl-2/Bax value in different activity groups.

Group (n = 5)	Radioactivity(MBq)	Tumor absorbeddose (Gy)	Pc-3 cell apoptosisrate (%) rate	SNK	Pc-3 cell necrosis(%)	SNK	Apo2.7 positive (%)	Caspase-3positive (%)	Bcl-2/Bax
14	0	0.00±0.00	0.67±0.15	A	1.8±0.5	A	4.9±1.8	7.69±1.5	2.07±0.85
9	0.37	2.89±0.20	10.09±1.84	B	2.2±0.6	B	8.3±2.4	10.43±2.6	1.64±0.62
10	0.74	5.78±0.25	17.36±4.33	C	6.0±1.1	B	13.6±2.3	13.46±2.9	1.02±0.52
11	1.48	11.56±1.29	21.85±3.04	D	11.7±0.5	C	15.0±3.2	14.92±4.1	0.81±0.32
12	2.96	23.12±3.12	33.67±3.69	E	13.1±1.0	D	16.3±3.1	18.68±4.6	0.37±0.12
13	5.92	46.23±4.20	27.76±4.09	F	23.4±2.6	D	27.3±4.9	25.57±5.3	0.07±0.03

Variance analysis after conversion of tumor cell apoptosis and necrosis rate to square root: cell apoptosis rate, F = 178.12, P<0.001, statistical difference between groups; Cell necrosis rate, F = 249.27, P<0.001, statistical difference between groups, if the variance analysis of the mean Pc-3 apoptosis and necrosis rate showed statistical differences, Student–Newman–Keuls’ method was employed.

Table 3 Rates of absorbed dose and Pc-3 apoptosis on same dosage (1.48 MBq) but between different time groups.

Group (n = 3)	Time (h)	Tumor absorbed dose (Gy)	Pc-3 apoptosis rate1(%)	SNK
15	6	7.25±2.58	10.00±3.02	A
16	12	13.73±2.43	17.93±2.24	B
17	24	24.82±5.14	33.85±4.54	C
18	36	46.06±8.55	27.85±3.04	D
19	48	47.43±11.60	18.41±5.40	E

¹Variance analysis after conversion of tumor cell apoptosis rate to square root: F = 87.76, P<0.001 statistical difference between groups.