Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Table 1 Cell culture media.

	DM	AM
	DMEM	DMEM/Ham’s F12
	20 mmol/L Hepes	20 mmol/L Hepes
	50 µg/mL gentamycin	50 µg/mL gentamycin
	50 mL/L human serum	50 mL/L human serum
		10 ng/mL EGF
		1 ng/mL HGF
		1 ng/mL GM-CSF
D-glucose	2 g/L	3.15 g/L
Galactose	2 g/L
Ornithine	0.1 g/L
L-proline	0.03 g/L	0.02 g/L
Nicotinamide	305 mg/L	2.02 mg/L
ZnCl2	0.544 mg/L
ZnSO4·7H2O	0.75 mg/L	0.432 mg/L
CuSO4·5H2O	0.2 mg/L	0.0013 mg/L
MnSO4	0.025 mg/L
L-glutamine	580 mg/L	365 mg/L
ITS1	0.5 g/L
Dexamethasone	1.0 g/L

¹ITS = rh-insulin 5.0 mg/L, human transferrin 5.0 mg/L, selenium 5.0 µg/L.