Adenosine 5'triphosphate transport and accumulation during the cold preservation of rat hepatocytes in University of Wisconsin solution

Table 1 Indices of the respiration activity (nmol O₂/min/10⁶ cells) in freshly isolated hepatocytes (controls) and cold preserved hepatocytes 72 h in UW solution without ATP (a-group) or with 10 mmol/L ATP (c-group). The cells from the different groups were resuspended and incubated at 37 °C for 120 min in KHR. Respiratory activity was determined at time 0, 60 and 120 min Data are expressed as the mean±SD for three separate preparations.

Indices	Freshly Isolated Hepatocytes (Controls) Incubated in KHR			Cold preserved Hepatocytes 72 h in UW without ATP (a-group).Incubated in KHR			Cold preserved Hepatocytes 72 h in UW with 10 mM ATP (c-group).Incubated in KHR
	0 min	60 min	120 min	0 min	60 min	120 min	0 min	60 min	120 min
Endogenous respiration(Vend)	336	304	285a	3210	175bd	124ade	353	214bd	213ad
Respiration Stimulated by succinate(Vsucc)	4910	5013	5015	599	5317	578	522	509	598
Vsucc/Vend	1.480.10	1.670.23	1.770.27a	1.940.45	3.410.81bcdf	4.420.96ad	1.490.10	2.100.30bdf	2.790.46ad

Note: V_succ/V_end are the ratios of the rates of respiration in the presence or absence of succinate. Data are given as the mean±SD for hepatocytes prepared from three rats for each group. Statistic differences inside groups. Indicates that endogenous respiration and V_succ/V_end from 120 min were different from 0 and 60 min (^aP<0.05); Indicates that endogenous respiration and V_succ/V_end from 60 min were different from 0 min (^cP<0.05). Indicates that Vsucc/Vend from 60 min was different from 120 min (^eP<0.05). Statistic differences between groups. Indicates that endogenous respiration and V_succ/V_end from a and c groups were different from controls at 60 and 120 min (^gP<0.05). Indicates that endogenous respiration from a group was different from c group (ⁱP<0.05). Indicates that V_succ/V_end from a and c groups were different (^kP<0.05).

Table 2 Total water content (intracellular and extracellular water space) of cold-stored hepatocytes under different conditions. Cells were cold stored (0 °C, 10 min) in UW solution containing ATP: 0.5, 1.0, 2.5, 5.0, 10 mmol/L and without ATP. Intracellular water space was determined as described in Materials and Methods. Data are given as the mean±SD for hepatocytes prepared from three rats for each group.

Extracellular concentration of	H2Ointracellular (mL/106 cells)	H2Oextracellular (mL/106 cells)
ATP (mmol/L)
–	5.47±0.27	0.10±0.03
0.5	6.35±0.91	0.11±0.03
1	5.43±0.63	0.09±0.01
2.5	5.77±0.87	0.12±0.04
5	5.48±0.33	0.11±0.04
10.0	6.10±0.34	0.13±0.03