Protective effects of 5,4’-dihydroxy-3’,5’-dimethoxy-7-O-β-D-glucopyranosyloxy-flavone on experimental hepatic injury

Table 1 Effects of RF on rat serum ALT, AST and Alb (mean±SD, n = 10).

Group	Dose(mg/kg)	ALT (U/L)	AST (U/L)	Alb (g/L)
Normal	–	48.22±21.08d	41.67±8.94d	49.78±4.75d
Model	–	177.74±19.72	147.22±7.44	37.08±3.19
Matrine	13.5	119.91±41.85b	98.52±26.36d	43.63±3.12d
RF	4.5	137.87±45.93a	133.41±25.96	39.15±4.45
	13.5	68.39±19.30d	118.04±30.90a	43.38±4.77b
	40.5	80.87±35.32d	95.63±36.90d	45.37±4.04d

^aP<0.05,

^bP<0.01,

^dP<0.001, vs model group.

Table 2 Effects of RF on whole-blood viscosity of rat in hepatic fibrosis model induced by thioacetamide (mean±SD, n = 10).

Group	Dose (mg/kg)	Whole-blood viscosity (P, mPa/s)
		Lower sheer rate 10 s	Medium sheer rate 60 s	High sheer rate 120 s
Normal	–	10.03±2.36d	5.12±0.95d	4.04±1.03d
Model	–	22.60±4.64	10.73±1.82	7.83±1.35
Matrine	13.5	12.28±2.72b	6.67±0.88d	5.28±0.71d
RF	4.5	14.44±1.74a	9.60±1.39	7.18±0.60
	13.5	16.34±1.76+	8.33±0.711b	6.55±0.40d
	40.5	14.44±1.17d	7.76±1.23d	5.96±0.78d

^aP<0.05,

^bP<0.01,

^dP<0.001 vs model group.

Table 3 Effects of RF on HA and Hyp of rat in hepatic fibrosis model induced by thioacetamide (mean±SD, n = 10).

Group	Dose (mg/kg)	HA (mg/L)	Hyp (mg/ g)
Normal	–	214.17±38.61b	40.42±10.60b
Model	–	521.96±70.17	192.04±38.29
Matrine	13.5	415.41±94.24b	145.20±47.21b
RF	4.5	354.17±59.90b	133.16±27.17b
	13.5	331.89±38.39b	106.83±21.11b
	40.5	339.19±49.63b	120.28±26.32b

^bP<0.001 vs model group.

Table 4 Effects of RF on ALT, AST and Alb (mean±SD, n = 8).

Group	Dose (mg/mL)	ALT (U/L)	AST (U/L)	Alb (g/L)
Normal	–	13.5±2.5d	14.1±3.3d	10.8±2.4b
Model	–	88.4±19.6	72.3±14.1	1.7±0.4
RF	5×10-4	59.3±10.7b	42.1±7.9d	7.3±1.3d
	5×10-5	45.1±7.6d	30.6±10.6d	5.5±0.9d
	5×10-6	69.2±19.1	55.0±10.4a	8.4±2.3d
	5×10-7	71.5±20.5	60.3±17.1	8.7±2.5d

^aP<0.05,

^bP<0.01,

^dP<0.001 vs model group.

Table 5 Effects of RF on SOD, GSH-Px and MDA (mean±SD, n = 8).

Group	Dose (mg/mL)	SOD (U/mg)	GSH-Px (U/mg)	MDA (nmol/mg)
Normal	–	10.1±0.8b	10.6±1.2b	4.6±0.7b
Model	–	4.2±0.7	3.8±0.6	19.0±2.4
RF	5×10-4	6.1±1.0b	7.6±0.9b	10.3±3.0b
	5×10-5	5.9±1.3b	9.1±1.3b	8.2±2.7b
	5×10-6	5.6±0.9b	6.8±1.1b	12.5±2.0b
	5×10-7	5.4±0.5b	6.2±1.0b	14.5±1.8b

^bP<0.001 vs model group.

Table 6 Effects of RF on survival rate of primary cultured rat hepatocytes (mean±SD, n = 3).

Group	Dose (mg/mL)	OD	Survival rate (%)
Normal	–	1.57±0.05d	91.64±3.16d
Model	–	0.82±0.05	49.65±3.05
RF	5×10-4	1.45±0.04d	84.81±2.35d
	5×10-5	1.39±0.03d	81.37±3.04d
	5×10-6	1.13±0.06b	66.48±2.58b
	5×10-7	0.96±0.03a	55.90±3.31

^aP<0.05,

^bP<0.01,

^dP<0.001 vs model group.