Effects of glutamine supplementation on splenocyte cytokine mRNA expression in rats with septic peritonitis

Table 1 Compositions of semipurified diet (g/kg).

Ingredients	GLN-supplemented	Without GLN
Casein	165	220
GLN	45	-
Total nitrogen	34.4	34.4
Corn starch	667	657
Soybean oil	44	44
Vitamin mixture1	10	10
Salt mixture2	35	35
Methyl-cellulose	30	30
Choline chloride	1	1
DL-methionine	3	3

¹The vitamin mixture contained the following (mg/g): thiamin hydrochloride 0.6, riboflavin 0.6, pyridoxine hydrochloride 0.7, nicotinic acid 3, calcium pantothenate 1.6, D-biotin 0.02, cyanocobalamin 0.001, retinyl palmitate 1.6, DL-α-tocopherol acetate 20, cholecalciferol 0.25, and menaquinone 0.005.

²The salt mixture contained the following (mg/g): calcium phosphate diabasic 500, sodium chloride 74, potassium sulphate 52, potassium citrate monohydrate 220, magnesium oxide 24, manganese carbonate 3.5, ferric citrate 6, zinc carbonate 1.6, cupric carbonate 0.3, potassium iodate 0.01, sodium selenite 0.01, and chromium potassium sulphate 0.55.

Table 2 Compositions of TPN solution (mL/L).

	GLN-supplemented	Without GLN
Glucose 50%	418	412
Fat emulsion 20%	50	50
Moriamin-SN 10%1	417	556
GLN	11	—
Infuvita2	8	8
NaCl 3%	35	35
KCl 7%	10	10
K3PO4 8.7%	10	10
Ca-gluconate	10	10
MgSO4	4	4
ZnSO4	2	2
Choline chloride (g)	1	1

¹From Chinese Pharmaceuticals, Taipei, Taiwan. Contents per deciliter: Leu 1250 mg, Ile 560 mg, Lys acetate 1240 mg, Met 350 mg, Phe 935 mg, Thr 650 mg, Trp 130 mg, Val 450 mg, Ala 620 mg, Arg 790 mg, Asp 380 mg, Cys 100 mg, Glu 650 mg, His 600 mg, Pro 330 mg, Ser 220 mg, Tyr 35 mg, and aminoacetic acid (Gly) 1570 mg.

²From Yu-Liang Pharmaceuticals, Taoyuan, Taiwan. Contents per milliliter: ascorbic acid 20 mg, vitamin A 660 IU, ergocalciferol 40 IU, thiamine HCl 0.6 mg, riboflavin 0.72 mg, niacinamide 8 mg, pyridoxine HCl 0.8 mg, D-panthenol 3 mg, dl-α-tocopheryl acetate 2 mg, biotin 12 μg, folic acid 80 μg, and cyanocobalamin 1 μg.