Are polymorphisms of N-acetyltransferase genes susceptible to primary liver cancer in Luoyang, China?

Table 1 Primer sequence of NAT1 and NAT2.

Primer	Position	Length (bp)	Primer sequence
NAT1a	­10-29	1158	5’-TTAGGAATTCATGGACATTGAAGCATATCTTGAAAGAAT-3’
NAT1b	1127-1148		5’-GCTTTCTAGCATAAATCACCAA -3’
NAT2a	1-30	895	5’-ATGGACATTGAAGCATATTTTGAAAGAATT-3’
NAT2b	867-895		5’-AAGGGTTTATTTTGTTCCTTATTCTAAAT-3’

Table 2 Goodness-of-fit test for Hardy-Weinberg law for genotype frequencies of NAT1 in control group.

Genotypes	Observed number	Expected number
NAT1*3/ *3	20	11
NAT1*3/ *4	30	40
NAT1*3/ *10	15	22
NAT1*3/ *14B	4	4
NAT1*4/ *4	50	37
NAT1*4/ *10	27	39
NAT1*4/ *14B	5	6
NAT1*10/ *10	19	10
NAT1*10/ *14B	4	3
Total	173	173

χ² = 7.24, P>0.05.

Table 3 Goodness-of-fit test for Hardy-Weinberg law for genotype frequencies of NAT2 in control group.

Genotypes	Observed number	Expected number
NAT2*4/*4	100	91
NAT2*4/*6	23	33
NAT2*4/*7	28	34
NAT2*6/*6	6	4
NAT2*6/*7	12	7
NAT2*7/*7	4	4
Total	173	173

χ² = 4.51, P>0.05.

Table 4 Genotype frequencies of NAT1 in case group and control group.

Genotypes	Cases (%)	Controls (%)	Total
NAT1*3/ *3	4 (4.2)	20 (11.5)	24
NAT1*3/ *4	24 (25.0)	30 (17.2)	54
NAT1*3/ *10	3 (3.1)	15 (8.6)	18
NAT1*3/ *14B	4 (4.2)	4 (2.3)	8
NAT1*4/ *4	30 (31.3)	50 (28.7)	80
NAT1*4/ *10	10 (10.4)	27 (15.5)	37
NAT1*4/ *14B	3 (3.1)	5 (2.9)	8
NAT1*10/ *10	14 (14.7)	19 (10.9)	33
NAT1*10/ *14B	4 (4.2)	4 (2.3)	8
Total	96 (100.0)	173 (100.0)	269

χ² = 11.86, P>0.05.

Table 5 Genotype frequencies of NAT2 in case group and control group.

Genotypes	Cases (%)	Controls (%)	Total
NAT2*4/*4	57 (59.4)	100 (57.8)	157
NAT2*4/*6	9 (9.4)	23 (13.3)	32
NAT2*4/*7	15 (15.6)	28 (16.2)	43
NAT2*6/*6	2 (2.1)	6 (3.5)	8
NAT2*6/*7	8 (8.3)	12 (6.9)	20
NAT2*7/*7	5 (5.2)	4 (2.3)	9
Total	96 (100.0)	173 (100.0)	269

χ² = 2.94, P>0.05.

Table 6 Frequency distribution of phenotypes of NAT1 and NAT2 in case and control group.

NAT phenotypes	Cases (%)	Controls (%)	Total
NAT1 rapid acetylator	31 (32.3)	64 (37.0)	95
NAT1 slow acetylator	65 (67.7)	109 (63.0)1	174
Total	96 (100.0)	173 (100.0)	269
NAT2 rapid acetylator	81 (84.4)	151 (87.3)	232
NAT2 slow acetylator	15 (15.6)	22 (12.7)2	37
Total	96 (100.0)	173 (100.0)	269

¹χ² = 0.598, P>0.05;

²χ² = 0.44, P>0.05.

Table 7 Interaction between occupational exposure and NAT1*10 allele.

Occupationalexposures	NAT1*10	Cases	Controls	OR	95%CI
		51	124	1
	+	11	34	0.79	0.37-1.67
+		15	15	2.43	1.11-5.34
+	+	7	5	3.4	1.03-11.22

χ² = 8.42, P = 0.004.

Table 8 Interaction between NAT2 genotypes and HBV infection.

Genotypes	HBV infection (-)		HBV infection (+)
	Cases	Controls	Cases	Controls
NAT2*4/*4	33	100	24	1
NAT2*4/*6	5	24	4	2
NAT2*4/*7	6	28	11	0
NAT2*6/*6	1	5	1	1
NAT2*7/*7	6	4	1	0
NAT2*6/*7	2	8	2	0

HBV infection (­): χ² = 8.26, P = 0.143; HBV infection (+): χ² = 11.13, P = 0.049.