In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells

doi:10.3748/wjg.v10.i8.1132

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Apr 15, 2004 (publication date) through Dec 21, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Apr 15, 2004; 10(8): 1132-1136
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1132

Table 1 Experimental groups and concentrations of drugs

Groups	Names and concentrations of drugs
I	RPMI1640
II	L-OHP 4 µg/mL
III_a	rhGH 50 ng/mL
III_b	rhGH 100 ng/mL
III_c	rhGH 200 ng/mL
III_d	rhGH 400 ng/mL
IV_a	rhGH 50 ng/mL + L-OHP 4 µg/mL
IV_b	rhGH 100 ng/mL + L-OHP 4 µg/mL
IV_c	rhGH 200 ng/mL + L-OHP 4 µg/mL
IV_d	rhGH 400 ng/mL + L-OHP 4 µg/mL

Table 2 Scheme of drugs in each group

Group	I	II	III_a	III_b	III_c	III_d	IV_a	IV_b	IV_c	IV_d
L-OHP (µL)	/	50	/	/	/	/	50.0	50	50	50
rhGH (µL)	/	/	12.5	25	50	100	12.5	25	50	100
RPMI1640 (µL)	150	100	137.5	125	100	50	87.5	75	50	/

Table 3 Effects of rhGH and L-OHP on BGC823 cells (n=4, mean ± SD)

Groups	OD values	Survival rate (%)	Inhibitory rate (%)
I	0.863 ± 0.172	100.00	0
II	0.425 ± 0.086^b	49.25^b	50.75^b
III_a	0.947 ± 0.142	109.73	-9.73
III_b	0.894 ± 0.220	103.59	-3.59
III_c	0.848 ± 0.346	98.26	1.74
III_d	0.844 ± 0.196	97.79	2.21
IV_a	0.338 ± 0.240^b	39.17^b	60.83^b
IV_b	0.318 ± 0.038^b	36.85^b	63.15^b
IV_c	0.318 ± 0.018^b	36.85^b	63.15^b
IV_d	0.306 ± 0.200^b	35.46^b	64.54^b

^bP<0.01 vs control group or rhGH group.

Table 4 Percentages of BGC823 cells in various phases of cell cycle (n = 3, mean ± SD)

Group	G₀–G₁(%)	S (%)	G₂–M(%)
I	47.75 ± 0.78	36.95 ± 0.49	15.25 ± 0.21
II	83.85 ± 1.77^b	7.85 ± 0.64^b	8.30 ± 1.13^b
III_a	53.03 ± 4.31	32.17 ± 7.47	14.73 ± 3.40
III_b	52.33 ± 4.55	32.63 ± 6.19	14.73 ± 1.88
III_c	52.33 ± 4.94	33.50 ± 8.17	14.17 ± 3.29
III_d	51.27 ± 5.94	33.67 ± 8.89	15.07 ± 3.07
IV_a	85.07 ± 2.62^b	6.67 ± 3.88^b	8.00 ± 1.82^b
IV_b	86.47 ± 2.07^b	5.47 ± 1.88^b	7.77 ± 0.40^b
IV_c	85.53 ± 0.47^b	7.87 ± 4.99^b	6.37 ± 5.61^b
IV_d	86.13 ± 2.87^b	4.20 ± 1.61^b	9.67 ± 1.27^b

^bP<0.01 vs control group or rhGH group.

Table 5 Proliferation index in each group (n = 3, mean ± SD)

Group	PI
I	52.25 ± 0.78
II	16.15 ± 1.77^b
III_a	46.97 ± 4.31
III_b	47.67 ± 4.55
III_c	47.67 ± 4.94
III_d	48.73 ± 5.94
IV_a	14.93 ± 2.62^b
IV_b	13.53 ± 2.07^b
IV_c	14.47 ± 0.47^b
IV_d	13.87 ± 2.87^b

^bP<0.01 vs control group or rhGH group.

Table 6 DNA inhibitory rate in rhGH group and rhGH+L-OHP group (n = 3, mean ± SD)

Group	DNA inhibitory rate (%)
III_a	111.1 ± 9.4
IV_a	178.1 ± 6.8
III_b	109.5 ± 9.8
IV_b	181.0 ± 4.0
III_c	109.5 ± 10.5
IV_c	178.2 ± 1.9
III_d	107.2 ± 12.4
IV_d	180.3 ± 7.6

Citation: Chen JY, Liang DM, Gan P, Zhang Y, Lin J. In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells. World J Gastroenterol 2004; 10(8): 1132-1136
URL: https://www.wjgnet.com/1007-9327/full/v10/i8/1132.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i8.1132