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©The Author(s) 2004.
World J Gastroenterol. Apr 15, 2004; 10(8): 1132-1136
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1132
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1132
Table 1 Experimental groups and concentrations of drugs
Groups | Names and concentrations of drugs |
I | RPMI1640 |
II | L-OHP 4 µg/mL |
IIIa | rhGH 50 ng/mL |
IIIb | rhGH 100 ng/mL |
IIIc | rhGH 200 ng/mL |
IIId | rhGH 400 ng/mL |
IVa | rhGH 50 ng/mL + L-OHP 4 µg/mL |
IVb | rhGH 100 ng/mL + L-OHP 4 µg/mL |
IVc | rhGH 200 ng/mL + L-OHP 4 µg/mL |
IVd | rhGH 400 ng/mL + L-OHP 4 µg/mL |
Table 2 Scheme of drugs in each group
Group | I | II | IIIa | IIIb | IIIc | IIId | IVa | IVb | IVc | IVd |
L-OHP (µL) | / | 50 | / | / | / | / | 50.0 | 50 | 50 | 50 |
rhGH (µL) | / | / | 12.5 | 25 | 50 | 100 | 12.5 | 25 | 50 | 100 |
RPMI1640 (µL) | 150 | 100 | 137.5 | 125 | 100 | 50 | 87.5 | 75 | 50 | / |
Table 3 Effects of rhGH and L-OHP on BGC823 cells (n=4, mean ± SD)
Groups | OD values | Survival rate (%) | Inhibitory rate (%) |
I | 0.863 ± 0.172 | 100.00 | 0 |
II | 0.425 ± 0.086b | 49.25b | 50.75b |
IIIa | 0.947 ± 0.142 | 109.73 | -9.73 |
IIIb | 0.894 ± 0.220 | 103.59 | -3.59 |
IIIc | 0.848 ± 0.346 | 98.26 | 1.74 |
IIId | 0.844 ± 0.196 | 97.79 | 2.21 |
IVa | 0.338 ± 0.240b | 39.17b | 60.83b |
IVb | 0.318 ± 0.038b | 36.85b | 63.15b |
IVc | 0.318 ± 0.018b | 36.85b | 63.15b |
IVd | 0.306 ± 0.200b | 35.46b | 64.54b |
Table 4 Percentages of BGC823 cells in various phases of cell cycle (n = 3, mean ± SD)
Group | G0–G1(%) | S (%) | G2–M(%) |
I | 47.75 ± 0.78 | 36.95 ± 0.49 | 15.25 ± 0.21 |
II | 83.85 ± 1.77b | 7.85 ± 0.64b | 8.30 ± 1.13b |
IIIa | 53.03 ± 4.31 | 32.17 ± 7.47 | 14.73 ± 3.40 |
IIIb | 52.33 ± 4.55 | 32.63 ± 6.19 | 14.73 ± 1.88 |
IIIc | 52.33 ± 4.94 | 33.50 ± 8.17 | 14.17 ± 3.29 |
IIId | 51.27 ± 5.94 | 33.67 ± 8.89 | 15.07 ± 3.07 |
IVa | 85.07 ± 2.62b | 6.67 ± 3.88b | 8.00 ± 1.82b |
IVb | 86.47 ± 2.07b | 5.47 ± 1.88b | 7.77 ± 0.40b |
IVc | 85.53 ± 0.47b | 7.87 ± 4.99b | 6.37 ± 5.61b |
IVd | 86.13 ± 2.87b | 4.20 ± 1.61b | 9.67 ± 1.27b |
Table 5 Proliferation index in each group (n = 3, mean ± SD)
Table 6 DNA inhibitory rate in rhGH group and rhGH+L-OHP group (n = 3, mean ± SD)
Group | DNA inhibitory rate (%) |
IIIa | 111.1 ± 9.4 |
IVa | 178.1 ± 6.8 |
IIIb | 109.5 ± 9.8 |
IVb | 181.0 ± 4.0 |
IIIc | 109.5 ± 10.5 |
IVc | 178.2 ± 1.9 |
IIId | 107.2 ± 12.4 |
IVd | 180.3 ± 7.6 |
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Citation: Chen JY, Liang DM, Gan P, Zhang Y, Lin J.
In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells. World J Gastroenterol 2004; 10(8): 1132-1136 - URL: https://www.wjgnet.com/1007-9327/full/v10/i8/1132.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i8.1132