Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

Table 1 Primers used for semi-quantitative RT-PCR and conditions of PCR reaction

Gene	Dir	Primer sequences (5’-3’)	Product size	Anne (°C)*	PCR cycles
S100A8	F	TGA CAA TGC CGT CTG AAC TG	271	60.4	28
	R	TCC TTG TGG CTG TCT TTG TG
S100A9	F	CAG CAT AAC CAC CAT CAT CG	310	60.1	28
	R	TTA CTT CCC ACA GCC TTT GC
EMP-1	F	AGT GCA AGC CTT CAT GAT CC	599	60.0	28
	R	TCC GAT CTG GGT CTC CAT AC
CSTB	F	GTC CCA GCT TGA ATC GAA AG	208	59.9	28
	R	ATC GTG CCT TTC TTT GTT GG
GAPDH	F	ACCACAGTCCATGCCATCAC	452	According to target gene
	R	TCCACCACCCTGTTGCTGTA

^{*F, forward; R, reverse; Dir, direction; Anne, annealing temperature.}

Table 2 Expression pattern of esophageal cancer deregulated genes during C57BL/6J mouse development

Gene name	Unigene	E11.5	E13.5	P1	Expression pattern	Change in ESCC
S100A8	Hs.100000	0.12	0.06	0.05	up-down-down	down
IGL	Hs.181125	0.22	0.09	0.08	up-down-down	up
RPL15	Hs.74267	0.23	0.08	0.07	up-down-down	down
EEF1A1	Hs.181165	3.50	0.72	0.43	up-down-down	down
TUBA1	Hs.75318	9.06	1.13	0.54	up-down-down	down
cystatin B	Hs. 695	0.19	0.05	0.07	up-down-down	down
ASN 120	Hs.239758	5.04	0.99	1.00	up-down-down	down
ASN 141	Hs.15087	2.56	0.74	0.79	up-down-down	down
ASN 409	Hs.238513	2.86	0.3	0.34	up-down-down	down
ASN 109	Hs.154390	2.52	0.61	0.59	up-down-down	down
SPRR2A	Hs.355542	0.24	0.48	0.67	down-up-up	down
RHCG	Hs.279682	0.19	0.11	0.78	down-down-up	down
keratin 4	Hs.3235	0.08	0.05	0.35	down-down-up	down
EMP1	Hs.79368	0.16	0.32	0.16	down-up-down	down

IGL, immunoglobulin lambda locus; RPL15, ribosomal protein L15; EEF1A1, eukaryotic translation elongation factor 1 alpha1; TUBA1, tubulin alpha1 (testis specific); ASN, automated sequencing number; SPRR2A, small proline-rich protein 2A; RHCG, Rh type C glycoprotein; EMP1, epithelial membrane protein 1.