Genotypes of Helicobacter pylori in patients with peptic ulcer bleeding

Table 1 Oligonucleotide primers used for cagA, vacA and iceA genotyping

Region detected	Primer designation	Primer sequence	Size of PCR product (bp)	References
s1 and s2	VA1-F	5’ATGGAAATACAACAAACACACC3’	259	21
	VA1-R	5’CTGCTTGAATGCGCCAAACTTTATC3’	286
s1a	SS1-F	5’GTCAGCATCACACCGCAAC3’	190	9
s1b	SS3-F	5’AGCGCCATACCGCAAGAG3’	187	9
s1c	S1C-F	5’CTYGCTTTAGTRGGGYTA3’	213	17
m1	VA3-F	5’GGTCAAAATGCGGTCATGG3’	290	9
	VA3-R	5’CCATTGGTACCTGTAGAAAC3’
m1T	m1T-F	5’GGTCAAAATGCGGTCATGG3’	290	21
	m1T-R	5’CTCTTAGTGCCTAAAGAAACA3’
m2	VA4-F	5’GGAGCCCCAGGAAACATTG3’	352	9
	VA4-R	5’CATAACTAGCGCCTTGCAC3’
iceA1	iceA1F	5’GTGTTTTTAACCAAAGTATC3’	247	16
	iceA1R	5’CTATAGCCASTYTCTTTGCA3’
iceA2	iceA2F	5’GTTGGGTATATCACAATTTAT3’	229	16
	iceA2R	5’TTRCCCTATTTTCTAGTAGGT3’
lcagA	lcagAD008	5’ATAATGCTAAATTAGACAACTTGAGCGA3’	297	5
	lcagAR008	5 ’TTAGAATAATCAACAAACATCACGCCAT3’

Table 2 Genotypes of Helicobacter pylori in patients with chronic gastritis, non-bleeding duodenal ulcers (DU), bleeding DU, non-bleeding gastric ulcers (GU) and bleeding GU n%

Diagnosis	No. of patients	No. of positive PCR	s1a	s1b	s1c	s2	m1	m1T	m2	cagA	iceA1	iceA2
Chronic gastritis	37	25	21(57)	0(0)	14(38)	0(0)	0(0)	8(22)	12(32)	22(59)	22(59)	2(5)
Non-bleeding DU	53	40	30(57)	4(8)	22(42)	0(0)	0(0)	11(21)	15(28)	29(55)	30(57)	3(6)
Bleeding DU	48	26	17(35)	4(8)	15(31)	2(4)	2(4)	3(6)	12(25)	25(52)	19(40)	7(15)
Non-bleeding GU	82	51	39(48)	0(0)	29(35)	0(0)	0(0)	10(12)	30(37)	41(50)	34(41)	13(16)
Bleeding GU	58	26	20(34)	1(2)	13(22)	0(0)	0(0)	1(2)	15(26)	22(38)	20(34)	4(7)

P > 0.05 vs variables between non-bleeding DU and bleeding DU, between non-bleeding GU and bleeding GU.

Table 3 Genotypes of Helicobacter pylori in patients with non-bleeding peptic ulcers and bleeding peptic ulcers n%

Diagnosis	No. of patients	No. of positive PCR	s1a	s1b	s1c	s2	m1	m1T	m2	cagA	iceA1	iceA2
Non-bleeding PU	135	91	69a(51)	4(3)	51(38)	0(0)	0(0)	21b(16)	45(33)	70(52)	64(47)	16(12)
Bleeding PU	106	52	37(35)	5(5)	28(26)	2(2)	2(2)	4(4)	27(25)	47(44)	39(37)	11 (10)

^aP = 0.017 between non-bleeding peptic ulcers and bleeding peptic ulcers.

^bP = 0.002 between non-bleeding peptic ulcers and bleeding peptic ulcers.