Apoptosis pathway of liver cells in chronic hepatitis

Table 1 Positive expression of apoptotic proteins in the liver specimens of chronic liver disease (%)

	n	Fas	FasL	Bax	Bcl-2	Bcl-XL	Bcl-2α
CH	72	63 (87.5)	68 (94.4)	66 (91.7)	41 (56.9)	44 (61.1)	28 (38.9)
LC	29	24 (82.8)	27 (93.1)	25 (86.2)	20 (69.0)	21 (72.4)	13 (44.8)

CH: Chronic hepatitis group. A: Fas: Bcl-2 χ² = 16.754, B: Fas: Bcl-X_Lχ² = 13.131; C: Fas: Bcl-2αχ² = 36.575, D: FasL: Bcl-2 χ² = 27.517; E: FasL: Bcl-X_Lχ² = 23.143, F: FasL: Bcl-2αχ² = 50.00; G: Bax: Bcl-2 χ² = 22.733, H: Bax: Bcl-X_Lχ² = 18.635; I: Bax: Bcl-2αχ² = 44.242; I: P < 0.001; J: Bcl-2: Bcl-2αχ² = 8.472, K: Bcl- X_L: Bcl-2αχ² = 7.111; J-K P < 0.01. LC: Liver Cirrhosis group. L: FasL: Bcl-2αχ² = 15.789; M: Bax: Bcl-2αχ² = 10.898; I-M: P < 0.001; N: Fas: Bcl-2αχ² = 9.032, P < 0.01; O: FasL: Bcl-2 χ² = 5.497; P: FasL: Bcl- X_Lχ² = 4.35; O-P: P < 0.05.

Table 2 Degree of expression of proapoptotic proteins on the liver specimens of chronic liver disease (%)

	n	Fas			FasL			Bax
		(+)	(++)	(+++)	(+)	(++)	(+++)	(+)	(++)	(+++)
CH	72	32 (44.4)	29 (40.3)	2 (2.8)	29 (40.3)	34 (47.2)	5 (6.9)	26 (36.1)	24 (33.3)	16 (22.2)
LC	29	13 (44.8)	9 (31.0)	2 (6.9)	8 (27.6)	15 (51.7)	4 (13.8)	11 (37.9)	14 (48.3)	0

Table 3 Degree of expression of anti-apoptotic proteins on the liver specimens of chronic liver disease (%)

	n	Bcl-2			Bcl-XL			Bcl-2α
		(+)	(++)	(+++)	(+)	(++)	(+++)	(+)	(++)	(+++)
CH	72	21 (29.2)	13 (18.1)	7 (2.8)	27 (37.5)	10 (13.9)	7 (9.2)	25 (34.7)	3 (4.2)	0
LC	29	12 (41.4)	6 (20.7)	2 (6.9)	12 (41.4)	7 (24.1)	2 (6.9)	8 (27.6)	9 (10.4)	2 (6.9)

Table 4 Apoptosis and mitochondria of the liver specimens of chronic liver disease

Code No.	HAI Score				Fas	Fasl	Bax	Bcl-2	Bcl-2α	Bcl-χl	A	B	A/B %
		G	S	HAI
1	CH-mild	1	0	1	++	++	++	++	+	-	3	20	15.0
2	CH-mild	1	0	4	+	++	+++	+++	-	+	2	26	7.7
4	CH-mild	1	1	3	++	+	+	+	-	++	2	26	7.7
7	CH-mild	1	0	6	++	+++	++	++	-	++	4	36	11.2
9	CH-mild	1	0	2	+	+++	-	-	-	+++	6	25	24.0
10	CH-mild	1	0	1	++	+	-	-	-	++	5	41	12.2
11	CH-mild	2	0	3	+	++	-	-	+	-	2	37	5.4
13	CH-mild	1	0	2	-	+	-	-	-	+	1	31	3.2
14	CH-mild	1	0	1	++	-	-	-	-	-	1	15	6.7
15	CH-mild	1	0	3	++	+	-	-	-	+	4	31	12.9
3	CH-MOD	2	0	8	++	+	+	+	+	+	8	40	20.0
5	CH-SEV	4	15	15	++	+++	-	-	-	-	2	36	5.6
6	LC	3	12	12	+	+	+++	+++	-	++	0	15
8	LC	4	15	15	-	-	+	+	-	+++	1	24	4.2
12	LC	4	8	8	+	+	-	-	-	++	5	34	14.7

MOD: Moderate; SEV: Severe; G: Grade; S: Stage. A: Number of hepatocytes with abnormal mitochondrial; B: Total number of hepatocytes examined by EM.