Basic Research
Copyright ©The Author(s) 2004.
World J Gastroenterol. Oct 15, 2004; 10(20): 3026-3033
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.3026
Table 1 PCR primer sequences for TNF-α, IL-1 β and IL-18 detection
CytokinePrimerSequenceReference
Human TNF- αupstreamTCA CAG GGC AAT GAT CCC AAA GTA GAC CTG C[2,3]
downstreamATG AGC ACT GAA AGC ATG ATC
Human IL-1 βupstreamTTA GGA AGA CAC AAA TTG CAT GGT GAA[2,3]
downstreamATG GCA GAA GTA CCT GAG CTC
Human IL-18upstreamGCT TGA ATC TAA ATT ATC AGT C[4]
downstreamGAA GAT TCA AAT TGC ATC TTA T
Human β -actinupstreamTTC CAG CCT TCC TTC CTG G[5]
downstreamTTG CGC TCA GGA GGA GCA AT