Targeting cyclooxygenase-2 with sodium butyrate and NSAIDs on colorectal adenoma/carcinoma cells

Table 1 PGE₂ (pg/10⁶ cells) production in HT-29 treated with Sodium butyrate and/or NSAIDs

Production of PGE2	2 mmol/L sodium butyrate	10 mmol/L aspirin	10 μmol/L NS - 398	Sodium butyrate + aspirin	Sodium butyrate + NS - 398
24 h	1306 ± 5.6	13.2 ± 4.1	35.2 ± 3.5	14.9 ± 1.9	45.9 ± 2.9
72 h	1427 ± 7.1	5.61 ± 2.7	5.95 ± 2.2	6.5 ± 1.0	8.49 ± 1.3

Table 2 Comparison of growth inhibition effects on HT-29 with Sodium butyrate alone or in combination with NSAIDs (%)

Time	2 mmol/L sodium butyrate	10 mmol/L aspirin	10 umol/L NS - 398	Sodium butyrate + aspirin	Sodium butyrate + NS - 398
24 h	4.28	55	10.2	58.3	26
72 h	23.7	72.2	63.9	81.9	70.5

Table 3 Percentage of HT-29 apoptotic cell rate (%)

	Control	Aspirin (mmol/L)				NS-398 (μmol/L)				Sodium butyrate (mmol/L)
Concentration		1	5	10	20	0.1	1	10	50	2	4	6
Apoptotic rate	4.2 ± 1.3	8.3 ± 1.6	10.9 ± 2.0	11.9 ± 2.7	29.7 ± 3.1	6 ± 1.4	7.8 ± 1.7	12.5 ± 3.1	19.5 ± 3.4	10.7 ± 2.5	15.9 ± 0.9	23.2 ± 4.1