A vaccinia replication system for producing recombinant hepatitis C virus

Table 1 Primers for RT-PCR

Oligodeoxynucleotide	5’ region	3’ region
Reverse transcription primers	AGCCCTGGCAGCGCCCCCTAGGGGGGCGCC (nt 791 to 762)	GGGGGATGGCCTATTGGCCTGGAGTGT (nt 9414 to 9388)
PCR primer 1	GCACAAATCCAAAACCCCAAAGAAAAA (nt 346-372)	CGCTCATCGGTTGGGGAGCAGGTAGATG (nt 9378 to 9351)
PCR primer 2	GACGAGCGGAATGTACCCCATGAGGTCGGC (nt 761-732)	CCATCCTTCTAGCCCAGGAGCAACTTGA (nt 8891 to 8918)

Table 2 Virion RNA quantitation in cell culture supernatants as measured by fluorescence quantitative RT-PCR (RNA copies per mL)

Culture time	RNA copies
	PT7HCV	PT7HCV + PvHCV	PvHCV	Control plasmid
48 h	1.12 ± 0.18 × 105	3.76 ± 0.22 × 105	0	0
72 h	4.08 ± 0.20 × 105	8.00 ± 0.28 × 105	0	0
96 h	1.28 ± 0.27 × 106	7.68 ± 0.22 × 106	0	0
120 h	3.24 ± 0.25 × 106	3.60 ± 0.18 × 107	0	0