Apoptosis of human primary gastric carcinoma cells induced by genistein

Table 1 A value of primary gastric cancer cells treated with different concentrations of genistein

	24 h	48 h	72 h
Control	0.400 ± 0.008	0.406 ± 0.007	0.404 ± 0.008
5 μmol/L genistein	0.361 ± 0.002a	0.334 ± 0.012b	0.305 ± 0.004b
10 μmol/L genistein	0.325 ± 0.004d	0.313 ± 0.003b	0.248 ± 0.004d
20 μmol/L genistein	0.308 ± 0.003d	0.249 ± 0.002d	0.206 ± 0.003d
40 μmol/L genistein	0.265 ± 0.004d	0.215 ± 0.004d	0.159 ± 0.002d

^aP < 0.05;

^bP < 0.01;

^dP < 0.001 vs control group.

Table 2 Apoptotic index (AI) of primary gastric cancer cells treated by genistein

	Control	24 h	48 h	72 h	96 h
AI (%)	1.25 ± 0.30	4.97 ± 0.80	18.44 ± 1.92	35.18 ± 0.35	43.93 ± 1.11
t		-6.13	-13.82	-180.16	-52.80
P		P < 0.05	P < 0.05	P < 0.05	P < 0.05

P < 0.05 vs control group; AI: Apoptotic index.

Table 3 Positivity rate (PR) of Bcl-2 in primary gastric cancer cells treated by genistein

	Control	24 h	48 h	72 h	96 h
PR(%)	36.34 ± 0.72	21.62 ± 0.08	10.60 ± 049	7.21 ± 0.45	4.54 ± 0.36
t		31.93	44.67	73.80	59.32
P		P < 0.01	P < 0.01	P < 0.01	P < 0.01

P < 0.01 vs control group; PR: Positivity rate.

Table 4 Positivity rate (PR) of Bax in primary gastric cancer cells treated by genistein

	Control	24 h	48 h	72 h	96 h
PR(%)	10.73 ± 0.57	20.63 ± 0.86	34.3 ± 0.81	45.96 ± 0.42	58.61 ± 1.46
t		-57.49	-41.23	-69.53	-51.31
P		P < 0.001	P < 0.001	P < 0.001	P < 0.001

P < 0.01 vs control group; PR: Positivity rate.