Evaluation of the viability and energy metabolism of canine pancreas graft subjected to significant warm ischemia damage during preservation by UW solution cold storage method

Table 1 Plasma glucose and IVGTT K value at the first week after transplantation and plasma insulin level in splenic and peripheral vein one hour after transplantation (mean ± SD)

Group	n	Plasma glucose (mol/L)	IVGTT K value	In splenic vein (mmol/L)	In peripheral vein (mmol/L)
1	3	5.6 ± 0.915	1.78 ± 0.1725	53.4 ± 7.135	8.6 ± 1.345
2	5	6.6 ± 0.9	1.58 ± 0.15	51.3 ± 8.2	8.1 ± 1.2
3a	6	6.7 ± 1.1	1.45 ± 0.12	50.6 ± 7.6	7.5 ± 1.1
3b	6	6.8 ± 0.8	1.42 ± 0.18	47.8 ± 7.6	7.5 ± 0.8
3c	6	11.9 ± 1.3	0.87 ± 0.16	35.0 ± 5.2	3.2 ± 0.7
3d	4	12.9 ± 1.8	0.60 ± 0.13	31.4 ± 8.1	2.7 ± 0.5

¹F = 36.9, P < 0.05;

²F = 32.9, P < 0.05;

³F = 7.38, P < 0.05;

⁴F = 38.2, P < 0.05;

⁵SNK test: between group 1, 2, 3a, 3b, P > 0.05; between group 3c, 3d, P > 0.05.

Table 2 Pancreatic juice flow during the first week after transplantation (mean ± SD, mL)

Group	n	Pancreatic juice flow/d
		1	2	3	4	5	6	7
2	5	27 ± 718	70 ± 1128	221 ± 1739	294 ± 3749	136 ± 2659	81 ± 2169	48 ± 1479
3a	6	24 ± 7	74 ± 17	216 ± 36	285 ± 36	138 ± 24	91 ± 19	53 ± 15
3b	6	24 ± 8	63 ± 15	204 ± 33	287 ± 43	142 ± 4	87 ± 22	41 ± 8
3c	6	12 ± 4	20 ± 6	68 ± 19	69 ± 19	83 ± 18	54 ± 12	30 ± 7
3d	4	7 ± 3	15 ± 8	12 ± 6	16 ± 6	8 ± 3	12 ± 5	10 ± 4

¹F = 9.87, P < 0.05;

²F = 25.9, P < 0.05;

³F = 67.4, P < 0.05;

⁴F = 88.2, P < 0.05;

⁵F = 45.2, P < 0.05;

⁶F = 15.9, P < 0.01;

⁷F = 13.2, P < 0.01;

⁸SNK test: between group 2, 3a, 3b, P > 0.05; between group 3c, 3d, P > 0.05;

⁹SNK test: between group 2, 3a, 3b, P > 0.05.

Table 3 Amylase in pancreatic juice of the first and the seventh day and viability after significant warm and cold preservation (mean ± SD)

Group	n	Amylase (μkat/L)		Functioninggrafts/rate (%)
		The first day	The seventh day
2	5	182 ± 4513	359 ± 2724	5/(100)
3a	6	183 ± 48	355 ± 37	6/(100)
3b	6	180 ± 42	327 ± 37	6/100)
3c	6	83 ± 24	29 ± 11	4/(66.7)5
3d	4	77 ± 30	28 ± 10	0/(0)5

¹F = 10.3, P < 0.05;

²F = 205.5, P < 0.05;

³SNK test: between group 2, 3a, 3b, P > 0.05;

⁴SNK test: between group 2, 3a, 3b, P > 0.05; between group 3c, 3d, P > 0.05.

⁵compare with Group 2, P < 0.05.

Table 4 Tissue concentration of ATP, ADP, AMP and TAN (mean ± SD, μmol/L)

	ATP	ADP	AMP	TAN
Group 1 (n = 3)	7.26 ± 0.5513	3.33 ± 0.20	1.49 ± 0.34	11.43 ± 1.3723
Group 2 (n = 5)	5.86 ± 0.52	1.01 ± 0.21	1.51 ± 0.26	7.93 ± 1.30
Group 3a (n = 6)	5.28 ± 0.37	1.31 ± 0.35	1.55 ± 0.35	8.02 ± 0.78
Group 3b (n = 6)	4.74 ± 0.41	2.01 ± 0.31	1.04 ± 0.24	7.36 ± 0.78
Group 3c (n = 6)	2.18 ± 0.21	0.83 ± 0.19	0.81 ± 0.23	4.04 ± 0.51
Group 3d (n = 4)	2.11 ± 0.17	0.86 ± 0.21	1.04 ± 0.25	3.33 ± 0.27

¹F = 17.0, P < 0.05;

²F = 23.9, P < 0.05;

³SNK test: between group 2, 3a, 3b, P > 0.05.