Transactivating effect of hepatitis C virus core protein: A suppression subtractive hybridization study

Table 1 Transient expression of pcDNA3.1(-)-core in HepG2 cells

Group	Coat Ag	P	P/N (n = 0.05)	Results (+/-)
pcDNA3.1(-)-core	pcDNA3.1(-)-core lysate	0.219	4.38	+
Blank plasmid	pcDNA3.1(-) lysate	0.034	0.68	-
Positive control	HCV core Ag	1.299	24.60	+
Negative control	PBS	0.012	0.24	-

Table 2 Comparison between positive clones and similar sequences in GenBank

High similarity to known genes	Number of similar clones
Ribosomal protein	21
Eukaryotic translation elongation factor	6
Eukaryotic translation initiation factor	5
Heat shock factor binding protein (HSBP)	5
Apolipoprotein	5
NADH dehydrogenase	3
Heterogeneous nuclear ribonucleoprotein	3
D-like (HNRPDL), transcript variant
Alpha-2-macroglobulin (A2M)	2
Bile acid-binding protein (BABP)	2
Cathepsin C (CTSC)	1
Fibronectin (FN precursor)	1
Magnesium-dependent protein phosphatase	1
Thioredoxin-related transmembrane protein	1
WEE1 gene for protein kinase	1