Construction and identification of recombinant vectors carrying herpes simplex virus thymidine kinase and cytokine genes expressed in gastric carcinoma cell line SGC7901

Table 1 Primer sequences of amplified genes

Genes	Primer sequences	Fragment length
TK	Forward: 5’-GC GAA TTC ATG GCT TCG TAC CCC TGC CAT C-3’	1 128 bp
	Reverse: 5’-GC GTT AAC TTA AGC CTC CCC CAT CTC CCG G-3
IL-2	Forward: 5’-GC CTC GAG ATG TAC AGG ATG CAA CTC CTG-3’	456 bp
	Reverse: 5’-GC GGA TTC TTA AGT CAG TGT TGA GAT GAT GC-3’
TNF-α	Forward: 5’-GC CTC GAG ATG GTC AGA TCA TCT TCT CGA AC -3’	474 bp
	Reverse: 5’-GC GGA TCC TTA CAG GGC AAT GAT CCC AAA -G-3’
IRES	Forward: 5’-GC GTT AAC AAT TCC GCC CCT CTC CCT CCC CC-3’	585 bp
	Reverse: 5’-GC CTC GAG AAT AGT AGC ACA AAA AGT TTC C-3’