Effect of 5-2Aza-2'-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2

Figure 4 Detection of aberrant methylation of the p16 gene in HCC cell line HepG2. Lane M: molecular weight marker; Lane 1: Neither treated with 5-aza-cdR nor digested with Hpa II or Msp I; Lane 2: Untreated with 5-aza-cdR, but digested with Hpa II; Lane 3: Untreated with 5-Aza-cdR, but digested with Msp I; Lane 4: Treated with 5-aza-cdR, but not digested with Hpa II or Msp I; Lane 5: Treated with 5-aza-cdR, and digested with Hpa II; Lane 6: Treated with 5-aza-cdR, and digested with Msp I.