Sugimoto M, Murata M, Yamaoka Y. Chemoprevention of gastric cancer development after Helicobacter pylori eradication therapy in an East Asian population: Meta-analysis. World J Gastroenterol 2020; 26(15): 1820-1840 [PMID: 32351296 DOI: 10.3748/wjg.v26.i15.1820]
Corresponding Author of This Article
Mitsushige Sugimoto, MD, PhD, AGAF, Professor, Department of Gastroenterological Endoscopy, Tokyo Medical University Hospital, 6-7-1, Nishishinjuku, Shinjuku-ku, Tokyo 1600023, Japan. sugimo@tokyo-med.ac.jp
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Meta-Analysis
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Mitsushige Sugimoto, Department of Gastroenterological Endoscopy, Tokyo Medical University Hospital, Sinjuku, Tokyo 1600023, Japan
Masaki Murata, Department of Gastroenterology, National Hospital Organization Kyoto Medical Center, Kyoto 6128555, Japan
Yoshio Yamaoka, Department of Gastroenterology, Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Oita 8795593, Japan
Author contributions: Sugimoto M acquisition of data, analysis and interpretation of data, drafting the article, final approval; Murata M acquisition of data, analysis and interpretation of data, final approval; Yoshioka Y design of the study, final approval.
Conflict-of-interest statement: The authors deny any conflict of interest.
PRISMA 2009 Checklist statement: This study was conducted according to the PRISMA agreement reporting guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Mitsushige Sugimoto, MD, PhD, AGAF, Professor, Department of Gastroenterological Endoscopy, Tokyo Medical University Hospital, 6-7-1, Nishishinjuku, Shinjuku-ku, Tokyo 1600023, Japan. sugimo@tokyo-med.ac.jp
Received: December 16, 2019 Peer-review started: December 16, 2019 First decision: January 7, 2020 Revised: March 15, 2020 Accepted: April 1, 2020 Article in press: April 1, 2020 Published online: April 21, 2020 Processing time: 126 Days and 18.8 Hours
Abstract
BACKGROUND
Helicobacter pylori (H. pylori) infection is a risk factor for gastric cancer (GC), especially in East Asian populations. Most East Asian populations infected with H. pylori are at higher risk for GC than H. pylori-positive European and United States populations. H. pylori eradication therapy reduces gastric cancer risk in patients after endoscopic and operative resection for GC, as well as in non-GC patients with atrophic gastritis.
AIM
To clarify the chemopreventive effects of H. pylori eradication therapy in an East Asian population with a high incidence of GC.
METHODS
PubMed and the Cochrane library were searched for randomized control trials (RCTs) and cohort studies published in English up to March 2019. Subgroup analyses were conducted with regard to study designs (i.e., RCTs or cohort studies), country where the study was conducted (i.e., Japan, China, and South Korea), and observation periods (i.e., ≤ 5 years and > 5 years). The heterogeneity and publication bias were also measured.
RESULTS
For non-GC patients with atrophic gastritis and patients after resection for GC, 4 and 4 RCTs and 12 and 18 cohort studies were included, respectively. In RCTs, the median incidence of GC for the untreated control groups and the treatment groups was 272.7 (180.4–322.4) and 162.3 (72.5–588.2) per 100000 person-years in non-GC cases with atrophic gastritis and 1790.7 (406.5–2941.2) and 1126.2 (678.7–1223.1) per 100000 person-years in cases of after resection for GC. Compared with non-treated H. pylori-positive controls, the eradication groups had a significantly reduced risk of GC, with a relative risk of 0.67 [95% confidence interval (CI): 0.47–0.96] for non-GC patients with atrophic gastritis and 0.51 (0.36–0.73) for patients after resection for GC in the RCTs, and 0.39 (0.30–0.51) for patients with gastritis and 0.54 (0.44–0.67) for patients after resection in cohort studies.
CONCLUSION
In the East Asian population with a high risk of GC, H. pylori eradication effectively reduced the risk of GC, irrespective of past history of previous cancer.
Core tip: No meta-analysis is available in the literature about the chemopreventive effects of Helicobacter pylori eradication therapy on the incidence of gastric cancer (GC) focused on East Asian populations living in geographical areas with high incidences of GC. We conducted a meta-analysis to reevaluate the prevention of Helicobacter pylori eradication therapy on the incidence of GC, irrespective of history of endoscopic or surgical treatment for GC.
Citation: Sugimoto M, Murata M, Yamaoka Y. Chemoprevention of gastric cancer development after Helicobacter pylori eradication therapy in an East Asian population: Meta-analysis. World J Gastroenterol 2020; 26(15): 1820-1840
Gastric cancer (GC) is one of the major cancers in the world, especially in East Asian countries such as Japan, South Korea, and China. Helicobacter pylori (H. pylori)-associated GC is caused by multifactorial and multistep process. The International Agency for Research on Cancer of the World Health Organization categorized H. pylori as a group I carcinogenic factor of GC in 1994[1,2]. Many clinical trials have shown that H. pylori infection is associated with an elevated risk of GC development not only in patients with atrophic gastritis and intestinal metaplasia alone, but also in patients already treated by resection of GC (Table 1). Due to the small sample size in each report, however, it remains unclear whether the risk of GC related to H. pylori is similar among patients with atrophy and intestinal metaplasia alone compared with post-resection patients.
With or without H. pylori infection, severe gastric mucosal atrophy and intestinal metaplasia are well-known risk factors for peptic ulcers as well as GC[3,4]. Several pathological reporting systems, including the Sydney system, its Houston-updated version, and the operative link on gastritis assessment system, as well as endoscopic reporting systems, such as the Kyoto classification of gastritis, are used to select patients at high risk for GC based on severity of pathological or endoscopic gastric mucosal atrophy and intestinal metaplasia[4-7]. In addition, the severity of gastric mucosal inflammation, atrophy, and intestinal metaplasia has been shown to correlate with H. pylori virulence factors (i.e., cagA, vacA and oipA)[8-11]. Because > 90% of H. pylori isolated from East Asian populations carry the cagA, which is associated with increased proliferation and pro-inflammatory and pro-apoptotic gene expression, and the vacA s1m1 type, which is associated with enhanced production of toxin with higher vacuolating activity[12,13], most East Asian populations infected with H. pylori are at higher risk for GC than H. pylori-positive European and United States populations. Thus, it is important to evaluate the association of H. pylori infection with GC risk in East Asian populations in order to formulate strategies to reduce the risk of GC.
Many clinical trials have shown that eradication of H. pylori infection reduces the risk of GC development (Table 1). Following H. pylori eradication therapy, a gradual decrease in severity of gastric atrophy in the gastric body and antrum and intestinal metaplasia in the body has been shown[14]. In 2012, the Japanese health insurance system began to cover H. pylori eradication treatment in patients with endoscopically-confirmed H. pylori-associated gastritis. International guidelines strongly recommend eradication of H. pylori in patients to prevent development of GC[15,16]. However, it remains unclear whether eradication therapy exerts the same preventive effect on GC among different groups, e.g., those of different nationalities, those with a history of gastritis, those with a prior history of GC resection, etc., with different risk levels for GC development.
In general, the risk of GC development differs between Western population and East Asian population, due to different life style, different genetics, and different H. pylori strain. Although it is important to evaluate the risk of GC separately for Western and East Asian population, previous meta-analysis did not evaluate this point and meta-analyzed all of studies, irrespective with nationalities. Thus, it is required to evaluate the association of H. pylori infection with GC risk in East Asian populations in order to formulate strategies to reduce the risk of GC. We conducted a meta-analysis to reevaluate the preventive effects of H. pylori eradication therapy on the incidence of GC, irrespective of history of endoscopic or surgical treatment for GC, especially in East Asian populations living in geographical areas with high incidences of GC.
MATERIALS AND METHODS
Search strategy and inclusion criteria
For a meta-analysis to investigate preventive effects of eradication therapy on GC development, we conducted a search of the medical literature using data of randomized control trials (RCTs) and cohort studies. Two researchers (MS and MM) independently searched both the PubMed and Cochrane Library databases using the terms “H. pylori,” “GC,” and “eradication therapy,” and reviewed titles and abstracts for all potential studies (Figure 1). The inclusion criteria were: (1) RCTs or cohort studies published in English up to March 2019; and (2) Studies comparing individuals receiving H. pylori eradication with those not receiving eradication treatment with respect to the incidence of primary GC in East Asian non-GC patients with gastritis or metachronous cancer in patients after resection of GC, irrespective with primary outcome or secondly outcome. All GC, including gastric dysplasia, was endosco-pically and pathologically diagnosed. Study included patients with GC or gastric dysplasia as gastric neoplasm was included as GC group in this meta-analysis. In non-GC patients with atrophic gastritis, the basement diseases before H. pylori eradication therapy were included atrophic gastric alone, peptic ulcer, gastric dysplasia and gastric adenoma. The full texts of potential studies were then screened to select studies meeting the inclusion criteria, and duplicated studies and multiple reports of the same study were excluded. When multiple articles were found, we used data from the latest publication date. Metachronous cancer was defined as a newly developed cancer at another site in the stomach > 1 year after resection for cancer. The exclusion criteria were (1) Non-East Asian patients; (2) Single-arm studies without a non-eradicated control group; and (3) Studies with an observation period < 1 year after eradication therapy.
Authors, publication year, country where the study was conducted, study design, numbers of treatment groups and control groups, number of patients developing GC, observation periods, patient conditions at baseline (sex and age), and pathological differentiation of GC were extracted from each study.
Statistical analysis
First, we divided studies into two kinds of clinical design: Non-GC patients with atrophic gastritis (at risk for primary GC) and patients after endoscopic and operative resection of early-stage GC (at risk for metachronous GC), because of their significantly different risk of cancer development. Subgroup analyses were conducted with regard to study designs (i.e., RCTs or cohort studies), country where the study was conducted (i.e., Japan, China, and South Korea), and observation periods (i.e., ≤ 5 years and > 5 years). The potential study bias in each study was evaluated by funnel plots. Heterogeneity was evaluated by I2 value and Cochran’s Q. The I2 value was used to assess the heterogeneity of the studies as follows: 0%–39%, low heterogeneity; 40%–74%, moderate heterogeneity; and 75%–100%, high heterogeneity. The relative risk (RR) and 95% confidence interval (CI) of each study were reported as the measure of effect size. All meta-analyses were conducted using open-source statistical software (Review Manager Version 5.3. Copenhagen: The Nordic Cochrane Centre, the Cochrane Collaboration, 2014). All P values were two-sided, and P < 0.05 was considered statistically significant. Calculations were performed using commercial software (SPSS version 20, IBM Inc; Armonk NY, United States).
RESULTS
Literature search and data extraction
A total of 38 studies which investigated the chemopreventive effects of H. pylori eradication on GC development among East Asian populations were included in the meta-analysis (Figure 1), consisting of 16 studies (4 RCTs and 12 cohort studies) in naïve non-GC patients with gastritis and 22 studies (4 RCTs and 18 cohort studies) in patients after resection for GC. Of 22 studies investigating the risks of metachronous cancers, a study from Cho et al[17] was included for post-surgical resection cases. In naïve non-GC patients with atrophic gastritis, the basement diseases before H. pylori eradication therapy were included atrophic gastric alone[18], peptic ulcer, gastric dysplasia[19-21] and gastric adenoma[22] or detail data not shown (but without history of GC)[23-26].
Finally, a total of 32775 non-GC cases with gastritis (5464 cases from RCTs and 27311 cases from cohort studies) and 9605 cases after resection for cancer (2007 cases for RCTs and 7598 cases from cohort studies) were included in this analysis (Table 1).
Four studies evaluated patients included GC or gastric dysplasia as gastric neoplasm[27-30]. The mean or median follow-up period ranged from 2.0–14.3 years, and the mean or median age of patients ranged from 46.7–79.2 years (Table 1). The ratio of males was from 46.2% to 98.1%. In most studies, H. pylori eradication therapy was performed using a regimen including proton pump inhibition (PPI) [i.e., omeprazole (20 mg, bid), lansoprazole (30 mg, bid), rabeprazole (10 or 20 mg, bid) or esomeprazole (20 mg, bid)] and two kinds of antimicrobial agents [i.e., clarithromycin (200, 400, or 500 mg, bid), amoxicillin (750 or 1000 mg, bid, or 500 mg, tid), and metronidazole (250 or 500 mg, bid)] (Table 1). In the RCTs, eradication rates were 66.2%–88.9% in studies for non-GC cases with gastritis and 75.0%–82.6% in cases after gastric resection (Table 1).
The median incidence of GC for the untreated control groups in non-GC cases of gastritis was 322.4 (68.7–1379.5) per 100000 person-years. Those in RCTs and cohort studies were 272.7 (180.4–322.4) and 467.2 (68.7–1379.5) per 100000 person-years, respectively (Table 2). In the treatment groups, median incidences of cancer in all studies, RCTs and cohort studies were 114.2 (0–588.2), 162.3 (72.5–588.2) and 113.7 (0–464.1) per 100000 person-years, respectively.
Table 2 Gastric cancer development and histological characteristics.
The median incidence of metachronous GC for the H. pylori-positive non-treated control groups after endoscopic and operative gastric resection was 2922.0 (406.5–10,166.0) per 100000 person-years. Those in RCTs and cohort studies were 1790.7 (406.5–2941.2) and 3117.9 (701.3–10166.0) per 100000 person-years, respectively (Table 2).
In the treatment groups, median incidence of metachronous GC in all, RCTs and cohort studies were 1229.2 (0–4333.1), 1126.2 (678.7–1223.1) and 1489.1 (0–4333.1) per 100000 person-years (Table 2).
Meta-analysis of GC risk in treated groups and untreated control group
In non-GC patients with gastritis, when combined with 4 RCTs[18,19,23,24] and 12 cohort studies, 180 (1.0%) patients developed GC among 17300 individuals who underwent eradication, and 285 (1.8%) patients did so among 15475 individuals in the untreated control group (Figure 2A). The RR for GC in the treatment groups compared with H. pylori-positive controls was 0.47 (95%CI: 0.38–0.59) (Figure 2A). No heterogeneity was found among the studies.
Figure 2 A forest plots for the incidence of gastric cancer between Helicobacter pylori-eradication group and non-eradication group in 16 studies combined with randomized control studies and cohort studies (A), 4 randomized control studies (B) and 12 cohort studies (C) in non-gastric cancer patients with atrophic gastritis.
There was no heterogeneity in the total analysis (I2 = 30%, P = 0.13), randomized control studies (I2 = 17%, P = 0.31) and cohort studies (I2 = 18%, P = 0.26). A: Randomized control studies + Cohort studies; B: Randomized control studies; C: Cohort studies.
In 4 RCTs[18,19,23,24], GC developed in 49 (1.8%) patients among 2733 H. pylori-negative treated cases, and in 73 (2.7%) patients among 2731 H. pylori-positive controls (Figure 2B). The mean RR comparing the H. pylori-negative eradicated group with the H. pylori-positive control group was 0.67 (95%CI: 0.47–0.96) (Figure 2B). No heterogeneity was found among the studies. In addition, in the cohort studies, the mean RR in the H. pylori-negative eradicated group was 0.39 (95%CI: 0.30–0.51) compared with the H. pylori-positive controls (Figure 2C).
In studies of a combination of 4 RCTs and 18 cohort studies of patients after endoscopic and operative resection, metachronous cancer occurred in 281 (6.0%) patients among 4688 treated individuals, and in 357 (7.3%) cases among 4917 cases in the H. pylori-positive control groups. The RR for metachronous cancer comparing H. pylori-negative treated groups with H. pylori-positive controls was 0.53 (95%CI: 0.44–0.64) (Figure 3A). No heterogeneity was found among the studies.
Figure 3 A forest plots for the incidence of metachronous gastric cancer between Helicobacter pylori-eradication group and non-eradication group in 22 studies combined with randomized control studies and cohort studies (A), 4 randomized control studies (B) and 18 cohort studies (C) in patients after endoscopic and operative resection.
No heterogeneity in the total analysis (I2 = 21%, P = 0.19), Randomized control studies (I2 = 0%, P = 0.40) and cohort studies (I2 = 28%, P = 0.13) was shown. A: Randomized control studies + Cohort studies; B: Randomized control studies; C: Cohort studies.
In the four RCTs[17,31-33], metachronous cancer occurred in 44 (4.4%) cases from 995 H. pylori-negative individuals, and in 88 (8.7%) cases among 1012 H. pylori-positive controls (Figure 3B). The RRs of the H. pylori-negative treated groups in RCTs and cohort studies were 0.51 (95%CI: 0.36–0.73) and 0.54 (0.44–0.67), respectively (Figure 3B and C).
Quality assessment
The funnel plot of all included studies did not suggest asymmetry in non-GC patients with atrophic gastritis and patients after resection of GC Supplementary Figure (1A and B), and statistical analysis with Egger’s test also confirmed that there was no publication bias according to different set of primary outcomes.
Subgroup analysis by different countries and observation periods
A total of 16 studies of cases of gastritis-10 from Japan, 5 from China, and 1 from South Korea—were analyzed (Figure 4A and B). The RR of the Japanese studies was 0.33 (95%CI: 0.25–0.45), which was lower than that from China (0.73; 0.51–1.03) and South Korea (0.51; 0.06–4.58) (Figs 4A and B). Seven Japanese and 15 Korean studies of post-gastric resection cases were conducted (Figure 4C and D). The RRs for metachronous cancer were similar between Japan (RR 0.57; 95%CI: 0.40–0.81) and South Korea (0.51; 0.42–0.63) (Figure 4C and D). When observation periods were subgrouped into ≤ 5 years and > 5 years, the RRs were similar between the two subgroups in studies of non-GC cases of gastritis, and in studies of cases after resection of GC (Figure 5A-D).
Figure 4 Forest plots of the incidence of gastric cancer between Helicobacter pylori-negative and positive groups with atrophic gastritis in studies published in different countries; 10 studies from Japan (A) and 5 from China (B).
Forest plots of the incidence of metachronous gastric cancer between Helicobacter pylori-negative and positive groups presenting with gastric cancer in 7 studies from Japan (C) and 15 from South Korea (D). A: Non-gastric cancer patients with atrophic gastritis: Japan; B: Non-gastric cancer patients with atrophic gastritis: China; C: Metachronous gastric cancer: Japan; D: Metachronous gastric cancer: South Korea.
Figure 5 Forest plots of the incidence of gastric cancer between Helicobacter pylori-negative and -positive groups with atrophic gastritis in studies of different observation period length, < 5 years (A) and > 5 years (B).
Forest plots of the incidence of metachronous gastric cancer in cases treated for gastric cancer in studies of different observation period length, < 5 years (C) and > 5 years (D). A: Non-gastric cancer with atrophic gastritis: within 5 years of resection; B: Non-gastric cancer with atrophic gastritis: more than 5 years after resection; C: Metachronous cancer: within 5 years of resection; D: Metachronous cancer: more than 5 years after resection.
Characteristics of GC after eradication therapy
Of non-GC patients with gastritis developing later cancer, 73.3% (85/116) of H. pylori-negative eradicated cases and 67.7% (126/186) of H. pylori-positive untreatedcontrols were observed be of the intestinal type (P = 0.31) (Table 2). Of patients developing metachronous cancers after endoscopic and operative resection of cancer, 84.0% (89/106) H. pylori-negative eradicated and 88.4% (122/138) H. pylori-positive non-treatment controls were observed to be of the intestinal type (P = 0.31) (Table 2).
DISCUSSION
In this meta-analysis, we evaluated whether H. pylori eradication therapy effectively reduced the risk of GC development in both non-GC patients with gastritis and patients after endoscopic and surgical resection for GC, especially in East Asian populations with high incidence rates of GC. This meta-analysis of 38 studies (16 studies in non-GC patients with gastritis and 22 in patients after gastric resection) showed that eradication therapy had a significant protective effect against development of GC with mean RRs of 0.47 (95%CI: 0.38–0.75) and 0.53 (95%CI: 0.44–0.64), irrespective of study design (RCTs or cohort studies), past history of GC, countries of origin, and observation periods after eradication therapy. This efficacy was similar to the previous meta-analysis performed by Yoon et al[34] (OR: 0.42; 95%CI: 0.32–0.56), Xiao et al[35] (RR: 0.50; 95%CI: 0.41–0.61), and Sugano[36] [OR: 0.46; 95%CI: 0.39–0.55]. Our observations suggest that H. pylori eradication therapy should be performed in all patients with H. pylori infection to prevent development of GC in East Asian populations.
Characteristics of GC risk in East Asian populations
Considered globally, East Asian countries, especially South Korea, have a high age-standardized incidence rate for GC. The age-standardized incidence rate per 100000 persons in 2018 was 39.6 in both sexes (57.8 in men and 23.5 in women) in South Korea, followed by 27.5 (40.7 and 16.0) in Japan and 20.7 (29.5 and 12.3) in China, vs a world rate in 2012 of 12.1 (17.4 and 7.5, respectively)https://(http://www.wcrf.org/dietandcancer/cancer-trends/stomach-cancer-statistics). As observed in global trends, however, in accordance with the increased chance of receiving H. pylori eradication therapy[37] resulting in a decrease in infection rates across the population[38] and improvement of sanitary conditions, incidence rates of GC are gradually declining, particularly H. pylori-associated non-cardia GC[37]. In Japan, although approximately 50000 GC deaths occurred annually over 40 years, deaths significantly decreased from 48427 in 2013 to 45509 in 2016 following expansion of insurance coverage for H. pylori eradication therapy[37]. In addition, national GC screening programs using upper endoscopy in South Korea and Japan may have contributed to the decrease of GC mortality by decreasing the risk of diagnosis at an advanced stage and by H. pylori eradication therapy after endoscopic diagnosis[39]. Currently, approximately half of all GCs in Japanese are detected at an early stage, confined to the mucosa or submucosa[40]. Establishment of an effective screening system for GC detection and the stratification of risk for GC development are considered to be important for reducing GC development and mortality.
Chemoprevention for metachronous GC
Endoscopic resection, including endoscopic mucosal resection and endoscopic submucosal dissection for early-stage GC, are widely accepted as curative[41]. With the development of treatment tools and endoscopy, endoscopic submucosal dissection is used as first-line treatment for early-stage GC in Japan and South Korea, because it enables en bloc resection of GC[42-44]. However, the risk of metachronous cancer after endoscopic resection is expected to be higher than the risk in non-GC patients with gastritis, and a drawback of endoscopic and operative resection is the residual risk of metachronous cancer arising from severe atrophic gastritis or intestinal metaplasia in the gastric remnant. In general, metachronous GCs are characterized as small, differentiated intramucosal cancers < 20 mm in size. The incidence rate of metachronous cancer within 3–5 years after endoscopic resection has been reported to be about 2.7%–15.6%[45]. The median incidence of metachronous cancer decreased to 1229.2 (0–4333.1) per 100000 person-years after eradication therapy from 2922.0 (406.5–10,166.0) in H. pylori-positive individuals in this meta-analysis. In a multicenter RCT by the Japan GAST Study Group in 2008, the cumulative incidence of metachronous cancer was 6.5% during 3-years follow-up. H. pylori eradication therapy decreased the incidence of metachronous cancer by approximately two-thirds in patients after endoscopic resection for early-stage GC (3.5% vs 9.6%, P = 0.003)[31]. Also in an RCT from South Korea, during a median follow-up of 5.9 years, metachronous cancer developed in 7.2% of patients who underwent successful eradication therapy and 13.4% of patients receiving placebo (HR: 0.50; 95%CI: 0.26–0.94), correlating with improvement in gastric corpus atrophy[32]. In this meta-analysis, only 36.4% of all studies (all 3 RCTs and five studies of 18 cohort studies in patients after endoscopic resection) showed significant reduction of metachronous cancer risk. This observation may have been caused by the small sample size in each report and it remains unclear whether the risk of GC related to H. pylori decrease after eradication therapy. The RRs with eradication therapy in RCTs and cohort studies were 0.51 (95%CI: 0.36–0.73) and 0.54 (0.44–0.67), respectively. Therefore, we recommend that all East Asian patients who remain H. pylori-positive after endoscopic resection for GC receive further eradication therapy to decrease the risk of metachronous cancer.
After gastrectomy, the cumulative incidence of metachronous cancer is 0.9%–3.0%, irrespective of H. pylori infection[45]. One report investigated the efficacy of eradication therapy for metachronous cancer risk after surgical partial gastric resection[17]. Cho et al[17] reported that eradication for patients after surgical resection is beneficial, as reflected by milder atrophy and intestinal metaplasia at 36 mo after surgery, but did not report any effect on GC. Therefore, although H. pylori in the gastric remnant would be associated with more severe atrophy and intestinal metaplasia over time, the efficacy of eradication therapy in patients with gastric remnants might be limited compared with that in non-GC patients with gastritis and patients after endoscopic gastric resection.
Subgroup analysis by different countries
Significant difference of GC risk after eradication among East Asian countries is shown in this meta-analysis. GC risk is known to depend on combination of bacterial factors (e.g., cagA status and vacA type), the host genetic factors (e.g., inflammatory cytokine genes, detoxification-related genes and oncogenes) and environmental factors (e.g., salt intake, smoking and alcohol)[46-51]. Therefore, the preventive effect for GC after eradication is also expected to depend on above different factors. Because the host genetic factors in East Asian populations (e.g., Japan, South Korea and China) is similar, different life style and different strain of H. pylori may cause difference for GC risk after eradication.
Chemoprevention for non-GC patients with gastritis
In this meta-analysis, although metachronous cancer incidence in H. pylori-positive patients after resection of GC was 2922.0 per 100000 person-years, that in non-GC patients with gastritis was not as high (322.4 per 100000 person-years). In 2001, Uemura et al[52] reported that GCs developed in 2.9% of H. pylori-positive patients at a mean follow-up of 7.8 years, but not in any H. pylori-negative patients or eradicated patients. This startling observation focused attention on the association of GC and H. pylori infection. This meta-analysis showed that 1 study of 4 RCTs (25.0%) and 7 studies of 12 cohort studies (58.3%) in East Asian populations had significant reduction in GC risk, with mean RRs of 0.67 (95%CI: 0.47–0.96) in RCTs and 0.39 (0.30–0.51) in cohort studies, suggesting that eradication for all H. pylori-positive non-GC patients with gastritis will decrease the risk of GC in East Asian populations. Further prospective studies with longer follow-up periods might help clarify the protective effect of H. pylori eradication against GC.
It was shown that the prophylactic effect of H. pylori eradication therapy was not observed in all groups of patients, but only in some ones. Longer follow-up after eradication therapy is associated with better prevention of GC development[53] and significant reduction in cancer incidence after eradication is observed only in pepsinogen test-negative subjects[25]. It may be important to clarify a high-risk group or a risk factor for GC development. A simple and safe method of identifying patients at increased risk of GC is necessary. As possible factors, pro-inflammatory cytokines (e.g. interleukin-1beta and tumor necrosis factor-alpha)[46,54], prostate stem cell antigen gene[55] methylation levels of any genes [e.g., miR-124a-3, empty spiracles homeobox 1 (EMX1) and NK6 homeobox 1 (NKX6-1)][56], and H. pylori virulence factors (e.g. cagA and vacA)[57,58], as well as endoscopic and pathological evidence of atrophy and intestinal metaplasia, have been cited. Ideally, eradication therapy should be instituted prior to the development of atrophy and intestinal metaplasia to achieve the optimal lowering of risk.
Incidence rate of GC after H. pylori eradication therapy between RCTs and cohort studies is the large difference: 272.7 (180.4–322.4) for RCT and 467.2 (68.7–1379.5) per 100000 person-years for cohort studies. This may be caused that patient background in each report is very heterogeneous in age, sex, disease, and severity of gastric atrophy and intestinal metaplasia. Further study will be required to clarify this problem.
High-efficacy eradication therapy and eradication regimens
In this meta-analysis, as summarized in Table 1, eradication therapy with a regimen including PPI and two kinds of antimicrobial agent (i.e., clarithromycin, amoxicillin, or metronidazole) in the RCTs resulted in eradication rates of 66.2%–88.9% in non-GC patients with gastritis and 75.0%–82.6% in patients after resection. To prevent GC, it is ideal to select an eradication regimen that provides a high eradication rate[59]. Unfortunately, however, because the frequent use of clarithromycin in general clinical situations has led to a global increase in the prevalence of clarithromycin-resistant strains, eradication rates with first-line clarithromycin-containing therapies are decreasing[60,61]. Recently, the Maastricht V/Florence Consensus Report has recommended quadruple therapy with or without bismuth, and without clarithromycin for patients living in areas with a high prevalence of clarithromycin resistance[62]. The new acid-inhibitory drug vonoprazan that produces rapid, strong and long-lasting gastric acid inhibition after administration of the first tablet in a dose-dependent manner has become clinically available in Japan, and vonoprazan-containing regimens have demonstrated effectiveness in patients infected with clarithromycin-resistant strains and patients living in areas where the prevalence of clarithromycin-resistant strains is > 15%[59,63-65]. As culture-based and pharmaco-genomics-based tailored treatment may have the potential to achieve an eradication rate exceeding 95%, the theoretical advantages of vonoprazan as evidenced by its potent acid inhibition during eradication therapy are considered to expedite risk reduction.
In addition, according to increase of incidence rates of antimicrobial agents-resistant H. pylori strains, patients refractory to eradication therapy including amoxicillin, clarithromycin, nitroimidazoles, fluoroquinolones, bismuth or tetracycline, is expected to be increasing. Recently, possible that use of new antimicrobial agents, such as rifabutin and sitafloxacin, have a potential with high eradication rate are suggested[66,67].
Limitations
We meta-analyzed studies in East Asian populations. Therefore, it is unclear whether this preventive strategy also applies to other populations. Studies investigating efficacy in populations with lower incidence rates of GC are scarce, and more investigation is needed. In addition, this meta-analysis is insufficient to investigate any association of preventive efficacy with patient age at the time of eradication. In an animal model with Mongolian gerbils, eradication therapy was shown to have an association with GC risk and duration of H. pylori infection, and a shorter duration of H. pylori infection due to early eradication was associated with a low risk of GC[68]. Long-term H. pylori infection may result in severe atrophic gastritis with intestinal metaplasia, and the earliest possible implementation of H. pylori eradication therapy is likely important in preventing GC.
In conclusion[17-33,52,53,69-94], this meta-analysis strengthens the evidence for the potential of H. pylori eradication therapy to reduce the risk of GC in East Asian populations with high incidence rates of GC. GC risk is known to depend on combination of bacterial factors (e.g., cagA status and vacA type), the host’s genetic factors (e.g., inflammation-related molecule polymorphism) and environmental factors (e.g., salt and smoking). Of those risk factors, because gastric mucosal change (e.g., gastric mucosal atrophy and intestinal metaplasia) with risk of GC development gradually progress after H pylori infection, best way is to avoid from infection of H. pylori, and if H. pylori infects, eradication therapy should be performed as soon as possible at younger age before progression of gastric mucosal atrophy in all H. pylori positive patients. The surveillance interval in patients after endoscopic and operative resection and non-GC patients with atrophic gastritis is important after eradication therapy. The National Comprehensive Cancer Network guidelines state that even for Tis or T1 with N0 lesions achieving R0 with endoscopic and surgical resection, all patients should be followed systematically, and that follow-up should include a complete history and physical examination every 3 to 6 mo for 1 to 2 years, every 6 to 12 mo for 3 to 5 years, and annually thereafter for all patients (http://www.nccn.org/ professionals/physician_gls/PDF/gastric.pdf).
ARTICLE HIGHLIGHTS
Research background
Helicobacter pylori (H. pylori) infection is a risk factor for gastric cancer (GC), especially in East Asian populations. Most East Asian populations infected with H. pylori are at higher risk for GC than H. pylori-positive European and United States populations. H. pylori eradication therapy reduces GC risk in patients after endoscopic and operative resection for GC, as well as in patients with atrophic gastritis. However, it remains unclear whether eradication therapy exerts the same chemopreventive effect on GC among different groups, e.g., those of different nationalities, those with a history of gastritis alone, those with a prior history of GC resection, etc., with different risk levels for GC development.
Research motivation
We hope to up-date evidences for preventive effect on development of GC after eradication therapy in East Asian populations.
Research objectives
To clarify the preventive effects of H. pylori eradication therapy for development of GC in an East Asian population with a high incidence of GC.
Research methods
PubMed and the Cochrane Library were searched for randomized control trials (RCTs) and cohort studies published in English up to March 2019 using the terms “H. pylori,” “GC,” and “eradication therapy”. Subgroup analyses were conducted with regard to study designs (i.e., RCTs or cohort studies), country where the study was conducted (i.e., Japan, China, and South Korea), and observation periods (i.e., ≤ 5 years and > 5 years). The heterogeneity and publication bias were also measured.
Research results
For patients with atrophic gastritis alone and patients after resection for GC, 4 and 4 RCTs and 12 and 18 cohort studies were included, respectively. In RCTs, the median incidence of GC for the untreated control groups and the treatment groups in cases of gastritis alone was 272.7 (180.4–322.4) and 162.3 (72.5–588.2) per 100000 person-years. In RCTs, the median incidence of metachronous GC for the untreated control groups and the treatment groups was 1790.7 (406.5–2941.2) and 1126.2 (678.7–1223.1) per 100000 person-years. Compared with non-treated H. pylori-positive controls, the eradication groups had a significantly reduced risk of GC, with a relative risk of 0.67 [95% confidence interval: 0.47–0.96] for patients with atrophic gastritis alone and 0.51 (0.36–0.73) for patients after resection for GC in the RCTs and 0.39 (0.30–0.51) and 0.54 (0.44–0.67) in cohort studies.
Research conclusions
The current meta-analysis showed that in the East Asian population with high incidence rates of GC, H. pylori eradication effectively reduced the risk of GC, irrespective of past history of previous cancer. The surveillance interval in patients after endoscopic and operative resection and patients with atrophic gastritis alone is important after eradication therapy.
Research perspectives
The results of the current meta-analysis may offer gastroenterologists and endoscopists more reliable evidence in efficacy of H. pylori eradication therapy and importance of surveillance after eradication therapy.
ACKNOWLEDGEMENTS
The authors thank Hitomi Mizuno, MD, PhD for checking data of each study and editing the paper.
Kodama M, Murakami K, Okimoto T, Sato R, Uchida M, Abe T, Shiota S, Nakagawa Y, Mizukami K, Fujioka T. Ten-year prospective follow-up of histological changes at five points on the gastric mucosa as recommended by the updated Sydney system after Helicobacter pylori eradication.J Gastroenterol. 2012;47:394-403.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 91][Cited by in F6Publishing: 95][Article Influence: 7.9][Reference Citation Analysis (0)]
Asaka M, Kato M, Takahashi S, Fukuda Y, Sugiyama T, Ota H, Uemura N, Murakami K, Satoh K, Sugano K; Japanese Society for Helicobacter Research. Guidelines for the management of Helicobacter pylori infection in Japan: 2009 revised edition.Helicobacter. 2010;15:1-20.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 282][Cited by in F6Publishing: 288][Article Influence: 20.6][Reference Citation Analysis (0)]
Wong BC, Lam SK, Wong WM, Chen JS, Zheng TT, Feng RE, Lai KC, Hu WH, Yuen ST, Leung SY, Fong DY, Ho J, Ching CK, Chen JS; China Gastric Cancer Study Group. Helicobacter pylori eradication to prevent gastric cancer in a high-risk region of China: a randomized controlled trial.JAMA. 2004;291:187-194.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 1015][Cited by in F6Publishing: 995][Article Influence: 49.8][Reference Citation Analysis (0)]
Wong BC, Zhang L, Ma JL, Pan KF, Li JY, Shen L, Liu WD, Feng GS, Zhang XD, Li J, Lu AP, Xia HH, Lam S, You WC. Effects of selective COX-2 inhibitor and Helicobacter pylori eradication on precancerous gastric lesions.Gut. 2012;61:812-818.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 110][Cited by in F6Publishing: 107][Article Influence: 8.9][Reference Citation Analysis (0)]
Ma JL, Zhang L, Brown LM, Li JY, Shen L, Pan KF, Liu WD, Hu Y, Han ZX, Crystal-Mansour S, Pee D, Blot WJ, Fraumeni JF, You WC, Gail MH. Fifteen-year effects of Helicobacter pylori, garlic, and vitamin treatments on gastric cancer incidence and mortality.J Natl Cancer Inst. 2012;104:488-492.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 314][Cited by in F6Publishing: 330][Article Influence: 27.5][Reference Citation Analysis (1)]
Zhou L, Lin S, Ding S, Huang X, Jin Z, Cui R, Meng L, Li Y, Zhang L, Guo C, Xue Y, Yan X, Zhang J. Relationship of Helicobacter pylori eradication with gastric cancer and gastric mucosal histological changes: a 10-year follow-up study.Chin Med J (Engl). 2014;127:1454-1458.
[PubMed] [DOI][Cited in This Article: ]
Yanaoka K, Oka M, Ohata H, Yoshimura N, Deguchi H, Mukoubayashi C, Enomoto S, Inoue I, Iguchi M, Maekita T, Ueda K, Utsunomiya H, Tamai H, Fujishiro M, Iwane M, Takeshita T, Mohara O, Ichinose M. Eradication of Helicobacter pylori prevents cancer development in subjects with mild gastric atrophy identified by serum pepsinogen levels.Int J Cancer. 2009;125:2697-2703.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 101][Cited by in F6Publishing: 107][Article Influence: 7.1][Reference Citation Analysis (0)]
Watanabe M, Kato J, Inoue I, Yoshimura N, Yoshida T, Mukoubayashi C, Deguchi H, Enomoto S, Ueda K, Maekita T, Iguchi M, Tamai H, Utsunomiya H, Yamamichi N, Fujishiro M, Iwane M, Tekeshita T, Mohara O, Ushijima T, Ichinose M. Development of gastric cancer in nonatrophic stomach with highly active inflammation identified by serum levels of pepsinogen and Helicobacter pylori antibody together with endoscopic rugal hyperplastic gastritis.Int J Cancer. 2012;131:2632-2642.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 66][Cited by in F6Publishing: 76][Article Influence: 6.3][Reference Citation Analysis (0)]
Fukase K, Kato M, Kikuchi S, Inoue K, Uemura N, Okamoto S, Terao S, Amagai K, Hayashi S, Asaka M; Japan Gast Study Group. Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial.Lancet. 2008;372:392-397.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 876][Cited by in F6Publishing: 889][Article Influence: 55.6][Reference Citation Analysis (0)]
El-Omar EM, Carrington M, Chow WH, McColl KE, Bream JH, Young HA, Herrera J, Lissowska J, Yuan CC, Rothman N, Lanyon G, Martin M, Fraumeni JF, Rabkin CS. Interleukin-1 polymorphisms associated with increased risk of gastric cancer.Nature. 2000;404:398-402.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 1690][Cited by in F6Publishing: 1624][Article Influence: 67.7][Reference Citation Analysis (0)]
Suzuki S, Muroishi Y, Nakanishi I, Oda Y. Relationship between genetic polymorphisms of drug-metabolizing enzymes (CYP1A1, CYP2E1, GSTM1, and NAT2), drinking habits, histological subtypes, and p53 gene point mutations in Japanese patients with gastric cancer.J Gastroenterol. 2004;39:220-230.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 51][Cited by in F6Publishing: 54][Article Influence: 2.7][Reference Citation Analysis (0)]
Ichikawa H, Sugimoto M, Uotani T, Sahara S, Yamade M, Iwaizumi M, Yamada T, Osawa S, Sugimoto K, Miyajima H, Yamaoka Y, Furuta T. Influence of prostate stem cell antigen gene polymorphisms on susceptibility to Helicobacter pylori-associated diseases: a case-control study.Helicobacter. 2015;20:106-113.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 12][Cited by in F6Publishing: 12][Article Influence: 1.3][Reference Citation Analysis (0)]
Asada K, Nakajima T, Shimazu T, Yamamichi N, Maekita T, Yokoi C, Oda I, Ando T, Yoshida T, Nanjo S, Fujishiro M, Gotoda T, Ichinose M, Ushijima T. Demonstration of the usefulness of epigenetic cancer risk prediction by a multicentre prospective cohort study.Gut. 2015;64:388-396.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 108][Cited by in F6Publishing: 100][Article Influence: 11.1][Reference Citation Analysis (0)]
Asaka M, Sugiyama T, Kato M, Satoh K, Kuwayama H, Fukuda Y, Fujioka T, Takemoto T, Kimura K, Shimoyama T, Shimizu K, Kobayashi S. A multicenter, double-blind study on triple therapy with lansoprazole, amoxicillin and clarithromycin for eradication of Helicobacter pylori in Japanese peptic ulcer patients.Helicobacter. 2001;6:254-261.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 115][Cited by in F6Publishing: 111][Article Influence: 4.8][Reference Citation Analysis (0)]
Malfertheiner P, Megraud F, O'Morain CA, Gisbert JP, Kuipers EJ, Axon AT, Bazzoli F, Gasbarrini A, Atherton J, Graham DY, Hunt R, Moayyedi P, Rokkas T, Rugge M, Selgrad M, Suerbaum S, Sugano K, El-Omar EM; European Helicobacter and Microbiota Study Group and Consensus panel. Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report.Gut. 2017;66:6-30.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 1710][Cited by in F6Publishing: 1855][Article Influence: 265.0][Reference Citation Analysis (1)]
Jenkins H, Sakurai Y, Nishimura A, Okamoto H, Hibberd M, Jenkins R, Yoneyama T, Ashida K, Ogama Y, Warrington S. Randomised clinical trial: safety, tolerability, pharmacokinetics and pharmacodynamics of repeated doses of TAK-438 (vonoprazan), a novel potassium-competitive acid blocker, in healthy male subjects.Aliment Pharmacol Ther. 2015;41:636-648.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 146][Cited by in F6Publishing: 205][Article Influence: 22.8][Reference Citation Analysis (0)]
Ribaldone DG, Fagoonee S, Astegiano M, Durazzo M, Morgando A, Sprujevnik T, Giordanino C, Baronio M, De Angelis C, Saracco GM, Pellicano R. Rifabutin-Based Rescue Therapy for Helicobacter pylori Eradication: A Long-Term Prospective Study in a Large Cohort of Difficult-to-Treat Patients.J Clin Med. 2019;8.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 38][Cited by in F6Publishing: 36][Article Influence: 7.2][Reference Citation Analysis (0)]
Uemura N, Mukai T, Okamoto S, Yamaguchi S, Mashiba H, Taniyama K, Sasaki N, Haruma K, Sumii K, Kajiyama G. Effect of Helicobacter pylori eradication on subsequent development of cancer after endoscopic resection of early gastric cancer.Cancer Epidemiol Biomarkers Prev. 1997;6:639-642.
[PubMed] [DOI][Cited in This Article: ]
Nakagawa S, Asaka M, Kato M, Nakamura T, Kato C, Fujioka T, Tatsuta M, Keida K, Terao S, Takahishi S, Uemura N, Kato T, Aiyama N, Saito D, Ssuzuki M, Imamura A, Sato K, Miwa H, Nomura H, Kaise M, Oohara S, Kawai T, Urabe K, Sakaki N, Ito S, Noda Y, Yanaka A, Kusugami K, Goto H, Furuta T, Fujino M, Kinjyo F, Ookusa T. Helicobacter pylori eradication and metachronous gastric cancer after endoscopic mucosal resection of early gastric cancer.Aliment Pharmacol Ther. 2006;24:214-218.
[PubMed] [DOI][Cited in This Article: ]
Shiotani A, Uedo N, Iishi H, Yoshiyuki Y, Ishii M, Manabe N, Kamada T, Kusunoki H, Hata J, Haruma K. Predictive factors for metachronous gastric cancer in high-risk patients after successful Helicobacter pylori eradication.Digestion. 2008;78:113-119.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 51][Cited by in F6Publishing: 54][Article Influence: 3.6][Reference Citation Analysis (0)]
Maehata Y, Nakamura S, Fujisawa K, Esaki M, Moriyama T, Asano K, Fuyuno Y, Yamaguchi K, Egashira I, Kim H, Kanda M, Hirahashi M, Matsumoto T. Long-term effect of Helicobacter pylori eradication on the development of metachronous gastric cancer after endoscopic resection of early gastric cancer.Gastrointest Endosc. 2012;75:39-46.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 134][Cited by in F6Publishing: 152][Article Influence: 12.7][Reference Citation Analysis (0)]
Seo JY, Lee DH, Cho Y, Lee DH, Oh HS, Jo HJ, Shin CM, Lee SH, Park YS, Hwang JH, Kim JW, Jeong SH, Kim N, Jung HC, Song IS. Eradication of Helicobacter pylori reduces metachronous gastric cancer after endoscopic resection of early gastric cancer.Hepatogastroenterology. 2013;60:776-780.
[PubMed] [DOI][Cited in This Article: ][Cited by in F6Publishing: 17][Reference Citation Analysis (0)]
Kato M, Nishida T, Yamamoto K, Hayashi S, Kitamura S, Yabuta T, Yoshio T, Nakamura T, Komori M, Kawai N, Nishihara A, Nakanishi F, Nakahara M, Ogiyama H, Kinoshita K, Yamada T, Iijima H, Tsujii M, Takehara T. Scheduled endoscopic surveillance controls secondary cancer after curative endoscopic resection for early gastric cancer: a multicentre retrospective cohort study by Osaka University ESD study group.Gut. 2013;62:1425-1432.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 158][Cited by in F6Publishing: 197][Article Influence: 17.9][Reference Citation Analysis (0)]
Bae SE, Jung HY, Kang J, Park YS, Baek S, Jung JH, Choi JY, Kim MY, Ahn JY, Choi KS, Kim DH, Lee JH, Choi KD, Song HJ, Lee GH, Kim JH. Effect of Helicobacter pylori eradication on metachronous recurrence after endoscopic resection of gastric neoplasm.Am J Gastroenterol. 2014;109:60-67.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 92][Cited by in F6Publishing: 95][Article Influence: 9.5][Reference Citation Analysis (0)]
You WC, Brown LM, Zhang L, Li JY, Jin ML, Chang YS, Ma JL, Pan KF, Liu WD, Hu Y, Crystal-Mansour S, Pee D, Blot WJ, Fraumeni JF, Xu GW, Gail MH. Randomized double-blind factorial trial of three treatments to reduce the prevalence of precancerous gastric lesions.J Natl Cancer Inst. 2006;98:974-983.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 310][Cited by in F6Publishing: 302][Article Influence: 16.8][Reference Citation Analysis (0)]
Kato M, Asaka M, Nakamura T, Azuma T, Tomita E, Kamoshida T, Sato K, Inaba T, Shirasaki D, Okamoto S, Takahashi S, Terao S, Suwaki K, Isomoto H, Yamagata H, Nomura S, Yagi K, Sone Y, Urabe T, Akamatsu T, Ohara S, Takagi A, Miwa J, Inatsuchi S. Helicobacter pylori eradication prevents the development of gastric cancer - results of a long-term retrospective study in Japan.Aliment Pharmacol Ther. 2006;24:203-206.
[PubMed] [DOI][Cited in This Article: ]
Take S, Mizuno M, Ishiki K, Nagahara Y, Yoshida T, Yokota K, Oguma K. Baseline gastric mucosal atrophy is a risk factor associated with the development of gastric cancer after Helicobacter pylori eradication therapy in patients with peptic ulcer diseases.J Gastroenterol. 2007;42 Suppl 17:21-27.
[PubMed] [DOI][Cited in This Article: ][Cited by in Crossref: 96][Cited by in F6Publishing: 93][Article Influence: 5.5][Reference Citation Analysis (0)]
