Mouse models for the discovery of colorectal cancer driver genes

Figure 2 Breeding scheme used to generate triple transgenic mice. Villin-Cre mice express the Cre recombinase enzyme from the gastrointestinal (GI)-specific villin promoter. T2/Onc mice carry a concatemer of T2/Onc transposons on Chromosome 1 or 15. Rosa-LSL-SB mice carry a knockin of the SB11 transposase enzyme downstream from a Lox-STOP-Lox (LSL) cassette at the Rosa locus. This design restricts T2/Onc transposition to the epithelial cells lining the GI tract.