Lesion discrimination with breath-hold hepatic diffusion-weighted imaging: A meta-analysis

Abstract

AIM: To investigate the diagnostic capability of breath-hold diffusion-weighted imaging (DWI) for differentiation between malignant and benign hepatic lesions.

METHODS: A total of 614 malignant liver lesions (132 hepatocellular carcinomas, 468 metastases and 14 intrahepatic cholangiocarcinomas) and 291 benign liver lesions (102 hemangiomas, 158 cysts, 24 focal nodular hyperplasia, 1 angiomyolipoma and 6 hepatic adenomas) were included from seven studies (eight sets of data).

RESULTS: The pooled sensitivity and specificity of breath-hold DWI were 0.93 [95% confidence interval (CI): 0.91-0.95] and 0.87 (95%CI: 0.83-0.91), respectively. The positive likelihood ratio and negative likelihood ratio were 7.28 (95%CI: 4.51-11.76) and 0.09 (95%CI: 0.05-0.17), respectively. The P value for χ² heterogeneity for all pooled estimates was < 0.05. From the fitted summary receiver operating characteristic curve, the area under the curve and Q* index were 0.96 and 0.91, respectively. Publication bias was not present (t = 0.49, P = 0.64). The meta-regression analysis indicated that evaluated covariates including magnetic resonance imaging modality, echo time, mean age, maximum b factor, and number of b factors were not sources of heterogeneity (all P > 0.05).

CONCLUSION: Breath-hold DWI is useful for differentiating between malignant and benign hepatic lesions. The diffusion characteristics of benign lesions that mimic malignant ones have rarely been investigated.

Key Words: Breath-hold imaging; Diffusion-weighted imaging; Hepatic tumor; Meta-analysis

Core tip: We investigated the diagnostic capability of breath-hold diffusion-weighted imaging (DWI) and found that it is useful for differentiating between malignant and benign hepatic focal lesions. The diffusion characteristics of the benign liver lesions that mimic malignant lesions have rarely been investigated and further studies are needed. Standardization of the acquisition protocol for breath-hold DWI across multicenter trials is recommended.

INTRODUCTION

Cancer is a leading cause of death worldwide, accounting for 8.2 million deaths in 2012 (Globocan 2012, International Agency for Research on Cancer). It is expected that annual cancer cases will rise from 14 million in 2012 to 22 million within the next two decades. Liver cancer killed 700000 people in 2008. Cancer mortality can be reduced if cases are detected and treated early through diagnosis and screening programs (http://www.who.int/cancer/events). Accurate diagnosis of focal hepatic lesions is essential for adequate treatment planning; in particular, to select patients who are candidates for hepatic resection, local ablation, or systemic chemotherapy[1-4].

Diffusion-weighted imaging (DWI) provides tissue contrast based on the diffusion properties of water molecules in tissue, without using any contrast agents. The inherent sensitivity of DWI sequences to motion remains a source of problems for liver imaging[5-7]. Respiratory motion degrades images through both temporal blurring and generation of discrete artifacts. Several techniques can be used to reduce the artifacts of respiratory motion: respiratory gating, respiratory ordered phase encoding, navigator gating, and signal averaging. None of these methods entirely eliminate the motion-associated degradation of image quality. Breath-hold imaging has proved to be far more satisfactory[8-10].

A review of the literature reveals that DWI is able to differentiate lesions with high water content (cysts and hemangiomas) from solid lesions. Differences in apparent diffusion coefficients have been reported between benign and malignant focal liver lesions[7,11-14]. Preliminary data are promising. The breath-hold technique is useful and considerably enhances magnetic resonance imaging (MRI). The present systematic review and meta-analysis aimed to investigate the diagnostic capability of breath-hold DWI for differentiating malignant and benign hepatic focal lesions.

MATERIALS AND METHODS

Search strategy

A computerized search was performed using PubMed (http://www.ncbi.nlm.nih.gov/pubmed/) including articles listed through April 2014. The following search terms were used: “liver and apparent diffusion coefficient (ADC)”, “liver and ADC”, “hepatic and ADC”, “hepatic and apparent diffusion coefficient”, “hepatic and DWI”, “liver and diffusion weighted imaging”, “liver and DWI”, “hepatic and diffusion weighted imaging”, and “hepatic and DWI”. The search was limited to English-language studies only. The reference lists of all included studies were examined for relevant publications.

Eligibility criteria for study selection

Studies were included in this analysis if: (1) breath-hold DWI was performed using either a 1.5T or 3.0T magnetic resonance (MR) scanner; (2) the diagnostic criteria of the malignant and benign hepatic focal lesions were clearly stated; (3) method of DWI analysis was reported; and (4) data were available to fill out cross-tabs in order to assess true-positive (TP), true-negative (TN), false-positive (FP) and false-negative (FN) cases.

Data collection

The characteristics of each study including study name, year of publication, MR modalities used, strength of field, pulse, repetition time (TR), echo time (TE), number of b factors, mean age, maximum b factor, mean size of malignant lesions, number of benign lesions [total, hemangiomas, cysts, focal nodular hyperplasia (FNH), angiomyolipoma and hepatic adenomas] and malignant lesions (total, hepatocellular carcinomas, metastases, and intrahepatic cholangiocarcinomas), TP, TN, FP, and FN, are shown in Tables 1 and 2.

Table 1 Liver breath-hold diffusion-weighted imaging studies and result.

No.	Ref.	MRI unit	Field(T)	Pulse	TR(ms)	TE(ms)	b factors(n)	b factor (Max)	PAT	Acceleration factor	Mean age (yr)	FS	Cutoff (ADC)	Lesion size (mal)
1	Erturk et al[15]	Philips	1.5	SS-SE-EPI	NA	120-125	2	1000	SENSE	2	60.4	Yes	1.63	2.3
2	Ichikawa T et al[16]	Siemens	1.5	SS-SE-EPI	NA	54	3	55	NA	NA	58.0	Yes	5.5	NA
3	Koh et al[18]	Phillips	1.5	SSEPI	1850	56	3	500	SENSE	2	57.0	NA	NA	1.96
4	Löwenthal et al[19]	Phillips	1.5	SSEPI	1850	68	2	500	SENSE	2	61.6	NA	Mal < 2.5 benign > 3	3
5	Taouli et al[20]	Phillips	1.5	SSEPI	2400	104	2	500	NA	NA	52.0	NA	1.5	5
6		Phillips	1.5	SSEPI	3106	104	4	400	NA	NA	52.0	NA	1.5	5
7	Yang et al[1]	Phillips	1.5	SSEPI	1338	66	3	800	SENSE	2	56.0	Yes	NA	1.76
8	Kim et al[17]	GE	1.5	SS-SE-EPI	NA	70	7	846	NA	NA	60.0	Yes	1.6	NA

ADC: Apparent diffusion coefficient; Mal: malignant; NA: Not available; PAT: Parallel acquisition technique; SENSE: Sensitivity encoding; SSEPI: Single-shot echo-planar imaging sequence; SS-SE-EPI: Single-shot spin-echo echo-planar imaging; T: Tesla.

Figure 1 Flow chart for articles identified and included in this meta-analysis.

Table 2 Liver breath-hold diffusion-weighted imaging studies and result, n.

No.	Ref.	Malignant				Benign						TP	FP	FN	TN
		Total	HCC	Met	Chol	Total	Hem	Cysts	FNH	Ang	Hep
1	Erturk et al[15]	42	21	21	0	44	16	28	0	0	0	40	4	2	40
2	Ichikawa et al[16]	63	48	15	0	11	11	0	0	0	0	59	0	4	11
3	Koh et al[18]	83	0	83	0	50	1	49	0	0	0	65	2.5	18	47.5
4	Löwenthal et al[19]	278	0	278	0	54	24	30	0	0	0	271	15	7	39
5	Taouli et al[20]	24	9	15	0	28	7	6	12	0	3	21	3	4	24
6		24	9	15	0	28	7	6	12	0	3	23	1	6	22
7	Yang et al[1]	51	12	26	13	46	19	27	0	0	0	49	5	2	41
8	Kim et al[17]	49	33	15	1	30	17	12	0	1	0	48	6	1	24

Ang: Angiomyolipoma; Chol: Cholangiocarcinoma; FN: False negative; FNH: Focal nodular hyperplasia; FP: False positive; HCC: Hepatocellular carcinoma; Hem: Hemangioma; Hep: Hepatic adenoma; Met: Metastases; TN: True negative; TP: True positive.

Figure 2 Forest plots. A: Sensitivity; B: Specificity; C: Positive likelihood ratio (LR); and D: Negative LR. CI: Confidence interval.

Statistical analysis

Statistical analyses were performed using Meta-DiSc version 1.4 or Stata 12.0 (StataCorp, College Station, TX, United States). Potential threshold effects were investigated using Spearman’s correlation coefficient. We assessed heterogeneity through visual inspection of the forest plots and with the I² statistic quantifying inconsistency across studies. For each study, the sensitivity, specificity, positive likelihood ratio (PLR) and negative likelihood ratio (NLR) was calculated (DerSimonian-Laird random effects model). A symmetric summary receiver operating characteristics (SROC) curve was fitted. Publication bias was evaluated by Deeks’ asymmetry test. To explore the sources of heterogeneity in the studies, we performed meta-regression analyses using the Moses-Shapiro-Littenberg method. P < 0.05 was considered to be statistically significant.

RESULTS

Study selection and data extraction

The initial database search identified 827 relevant articles that were published through April 2014. The initial screening by one reviewer reduced the total to 28. Finally, we selected eight sets of data in seven articles that met all the inclusion criteria for meta-analysis (Figure 1).

Description of studies

This meta-analysis was performed on a per-lesion basis. A total of 614 malignant liver lesions (132 hepatocellular carcinomas, 468 metastases and 14 intrahepatic cholangiocarcinomas) and 291 benign liver lesions (102 hemangiomas, 158 cysts, 24 FNH, one angiomyolipoma and six hepatic adenomas) were included (No.1-8; Table 1)[1,15-20]. The mean age of patients was 57.1 years.

All studies used a 1.5T MR scanner with single-shot echo-planar imaging sequence (No.1-8). Seven studies (No.1, 3-8) used a sequence with maximum b factor in the range of 400-1000 ms, while one study used a sequence with maximum b factor of 55 (No.2). Typical acquisition parameters include TE (No.1-8) of ≥ 54 ms (range: 56-125 ms) and TR of ≥ 1338 ms (range: 1338-3106 ms) (No.3-7). Three studies did not provide information on TR (No.1, 2, 8). Four studies did not provide information on the fat-suppressed technique (No.3-6). The parallel acquisition technique was used in four studies (No.1, 3, 4, 7) and the typical acceleration factor was 2. The results of all analyses are reported in Tables 1 and 2.

Synthesis of general diagnostic parameters

Figure 2 shows the forest plots of sensitivity (Figure 2A), specificity (Figure 2B), PLR (Figure 2C), and NLR (Figure 2D) of breath-hold DWI for differential diagnosis between focal malignant and benign hepatic lesions. The threshold effect was not present (P = 0.058).

The pooled sensitivity and specificity of breath-hold DWI were 0.93 [95% confidence interval (CI): 0.91-0.95] and 0.87 (95%CI: 0.83-0.91), respectively. PLR and NLR were 7.28 (95%CI: 4.51-11.76) and 0.09 (95%CI: 0.05-0.17), respectively. The P value for χ² heterogeneity for all pooled estimates was < 0.05.

The overall accuracy was further explored by drawing SROC curves, and the area under the curve (AUC) and Q* index (Figure 3) were 0.96 and 0.91, respectively, indicating good diagnostic accuracy. Publication bias was not present (t = 0.49, P = 0.64) (Figure 4).

Figure 3 Summary receiver operating characteristics curve. Sensitivity and specificity are plotted for individual studies. AUC: Area under the curve. SROC: Summary receiver operating characteristics.

Figure 4 There is no significant publication bias.

The meta-regression analysis indicated that evaluated covariates, including MRI modality, TE, mean age, maximum b factor, and number of b factors, were not sources of heterogeneity (all P > 0.05).

DISCUSSION

DWI has a strongpoint in that it provides excellent lesion-to-liver contrast with the suppression of the background signal of liver parenchyma as well as vessels, which reduces the likelihood of overlooked lesions[7,21,22]. Malignant tumors with hypercellularity, narrowed intercellular spaces, and increased density of cell membranes that hamper water molecule diffusion may well exhibit increased signal intensity on DWI[22]. Breath-hold imaging has proved to be more satisfactory. We used commonly available MRI techniques (e.g., no respiratory triggering) so that our results are applicable to most MRI units and not restricted to major academic centers[15].

Based on calculations of the relevant data available in the current published articles, our systematic review and meta-analysis demonstrated that breath-hold DWI was useful for differentiating between malignant and benign hepatic focal lesions. The results demonstrated that the overall diagnostic performance of the test with DWI to differentiate malignant and benign hepatic focal lesions was high. However, significant heterogeneity among studies was noted in our analysis.

Our meta-regression analysis indicated that evaluated covariates were not sources of heterogeneity. These results are consistent with recent systematic reviews[23], which have reported that neither threshold effect nor evaluated covariates including MR scanner, scanning technique, TR, TE, maximum b factor, number of b factors used for ADC calculation, mean tumor size, and mean patient age, were sources of heterogeneity. It is known that the best acquisition strategies for DWI sequences in focal liver disease are still a matter of debate. There was considerable variation in the results, which may be an indicator that more detailed investigation should be carried out on the presence of heterogeneity.

ADCs tend to decrease in the order of cysts, hemangiomas, HCCs, and metastases[24]. The malignant lesions, including metastases and HCCs, had the lowest ADCs, whereas the benign lesions, including hemangiomas and cysts, had the highest ADCs. Benign hepatocellular lesions had intermediate ADCs[20]. FNH and hepatic adenoma readily mimic malignant hepatic tumors, and these benign lesions often show increased signal intensity on DWI. However, the diffusion characteristics of the benign hepatocellular lesions, including cases of FNH (24/291) and adenoma (6/291), have rarely been reported and need further studies. It is known that DWI is more useful with hepatic metastases than with HCCs, primarily because the T2 relaxation time is long enough with most metastases, and there is no resemblance of histopathologic architecture between metastases and surrounding liver parenchyma[22]. However, the relevant data available for malignant hepatic focal lesions in the current published articles focus on hepatic metastases (468/614). All these data have demonstrated that the diagnostic capability of breath-hold DWI for differentiation of malignant and benign hepatic focal lesions might be overestimated.

Asymmetrical funnel plots are linked to publication bias, although there are other sources of asymmetry that have to be considered, including other dissemination biases, differences in the quality of smaller studies, presence of true heterogeneity, and chance[25-28]. In the present meta-analysis, the funnel plot indicated that there may not have been publication bias.

The present study had several limitations. First, there was notable heterogeneity among the studies. Evaluated covariates were not the sources and this needs further investigation. Second, diagnostic capability might be overestimated due to the possibility of selection bias. The diffusion characteristics of the benign liver lesions (e.g., FNH and adenoma) that mimic malignant lesions have rarely been investigated and require further studies.

In conclusion, breath-hold DWI was useful for differentiation between malignant and benign hepatic focal lesions. However, diagnostic capability might be overestimated due to the possibility of selection bias. Standardization of the acquisition protocol for breath-hold DWI across multicenter trials is recommended.

COMMENTS

Background

Diffusion-weighted imaging (DWI) provides tissue contrast based on the diffusion properties of water molecules in tissue, without using any contrast agents. The inherent sensitivity of DWI sequences to motion remains a problem for liver imaging. Breath-hold DWI has proven to be more satisfactory.

Research frontiers

There is no current consensus on the diagnostic capability of hepatic breath-hold DWI. We conducted a systematic review to investigate the diagnostic capability of breath-hold DWI for differentiating between malignant and benign hepatic focal lesions.

Innovations and breakthroughs

The diffusion characteristics of the benign liver lesions that mimic malignant lesions have rarely been investigated and need further study. Standardization of the acquisition protocol for breath-hold DWI across multicenter trials is recommended.

Applications

Breath-hold DWI was useful for differentiation of malignant and benign hepatic focal lesions.

Terminology

DWI provides tissue contrast based on the diffusion properties of water molecules in tissue. DWI plays a potential role in the differentiation and evaluation of liver tumors on the basis of high contrast between the lesion and normal tissue.

Peer-review

The paper discusses the prognostic value of DWI in differentiation of benign versus malignant hepatic masses. The meta-analysis is comprehensive and carefully done.