Stress-induced intestinal necrosis resulting from severe trauma of an earthquake

Abstract

AIM: To investigate the possible reasons and suggest therapeutic plan of stress-induced intestinal necrosis resulting from the severe trauma.

METHODS: Three patients in our study were trapped inside collapsed structures for 22, 21 and 37 h, respectively. The patients underwent 3-4 operations after sustaining their injuries. Mechanical ventilation, intermittent hemodialysis and other treatments were also provided. The patients showed signs of peritoneal irritation on postoperative days 10-38. Small intestinal necrosis was confirmed by emergency laparotomy, and for each patient, part of the small bowel was removed.

RESULTS: Two patients who all performed 3 operations died of respiratory complications on the first and second postoperative days respectively. The third patient who performed 4 operations was discharged and made a full recovery. Three patients had the following common characteristics: (1) Multiple severe trauma events with no direct penetrating gastrointestinal injury; (2) Multiple surgeries with impaired renal function and intermittent hemodialysis treatment; (3) Progressive abdominal pain and tenderness, and peritoneal irritation was present on post-traumatic days 10-38; (4) Abdominal operations confirmed segment ulcer, necrosis of the small intestine, hyperplasia and stiffness of the intestinal wall; and (5) Pathological examinations suggested submucosal hemorrhage, necrosis, fibrosis and hyalinization of the vascular wall. Pathological examinations of all 3 patients suggested intestinal necrosis with fistulas.

CONCLUSION: Intestinal necrosis is strongly asso-ciated with stress from trauma and post-traumatic complications; timely exploratory laparotomy maybe an effective method for preventing and treating stress-induced intestinal necrosis.

Key Words: Intestinal necrosis; Stress; Trauma; Earthquake; Exploratory laparotomy; Fatty acid binding protein

INTRODUCTION

Intestinal necrosis is a common condition that is frequently seen in clinical practice[1-6]. It is generally associated with mesenteric thrombosis or bacterial infection after eating contaminated foods[5,6]. However, few cases of stress-induced intestinal necrosis have been reported. Although animal studies have confirmed that stress can cause intestinal mucosal barrier disturbances[7,8], stress-induced intestinal necrosis has not been confirmed. We analyzed the clinical data of 3210 patients who were admitted to our hospital after the Wenchuan earthquake on May 12, 2008. In the laparotomy examinations of 3 patients with no penetrating abdominal trauma, intestinal necrosis was confirmed on postoperative days 10-38. Two of these patients died, while 1 survived and made a full recovery. For these 3 cases, we made the diagnosis of “stress-induced intestinal necrosis” after repeated careful consideration.

MATERIALS AND METHODS

The 3 male patients were 27, 36 and 42 years old, respectively (average age of 35 years). They were trapped inside collapsed structures for 22, 21 and 37 h, respectively (average time of 26.3 h). They were admitted into our hospital at 37, 44 and 39 h, respectively, after trapped in the buildings. Their primary earthquake-related traumatic injuries mainly occurred on the head and extremities, including 1 case with a left thorax crush injury. The physician specialists in various departments of our hospital consulted and discussed the cases with experts from The Liberation Army General Hospital of Beijing immediately after the patients’ admission. Treatment plans were designed individually by a multi-disciplinary team. The patients underwent 4, 3 and 3 surgeries, respectively, according to their clinical features. The final operation for each patient, which was a small bowel resection, was performed on postoperative days 38, 21 and 10, respectively. Antibiotic therapy, intravenous fluid hydration, mechanical ventilation and intermittent hemodialysis were administered to all 3 patients after admission.

Representative case report

A 27-year-old healthy male patient with a relatively healthy medical history had multiple body injuries due to high-force impacts from a building collapse in the Wenchuan earthquake on May 12, 2008. He was rescued after being trapped in building debris for 22 h. He was admitted to our hospital on May 14. He remained conscious and presented with multiple contusions on the head and both lower extremities at the time of admission. Neither of his legs could be moved freely, and both were significantly swollen. The pulse of the bilateral dorsalis pedis was absent. The patient’s toes presented as dark purple with poor blood circulation. A physical examination showed no signs of abdominal trauma. At the time of admission, emergency decompression surgery was performed on the osteofascial compartment of the left lower extremities under local anesthesia. We suspected that the patient had acute renal failure due to a lack of urination, and hemodialysis was administrated. On May 16, the patient underwent emergency amputation of both legs at the mid-thigh. On May 20, the patient experienced abdominal distension with diarrhea; blood appeared in a stool sample. On May 24, the patient developed worse abdominal distention, mild tenderness around the belly button and no rebound tenderness or muscle tension. Bowel sounds were absent. An ultrasound test revealed intestinal expansion and a small amount of peritoneal fluid. On May 26, jejunum drainage was placed under gastroscopy. The drainage output was 1000 mL, and the abdominal distension was significantly relieved. On May 30, the patient developed infections in right lower extremitie. A second amputation operation was performed on his right thigh. On June 3, thoracentesis was performed on his left chest cavity due to significantly increased pleural effusion. On June 7, the patient developed dark bloody stool that occurred 3-4 times per day. The patient’s urine output gradually returned to normal by intermittent hemodialysis. On June 19, the patient developed worsening abdominal pain and tenderness, and a mass was noted around the middle and lower abdomen. Peritoneal irritation was obviously present. On emergency abdominal exploration (Figure 1), significant intestinal adhesion was noted. The intestine approximately 210 cm below the Treitz ligament and 50 cm above the ileocecal valve was expanded, thickened and twisted into a mass, with a large amount of inflammatory exudate. Intestinal adhesion, necrosis and perforation with fistula were noted (Figure 2). The proximal jejunum was expanded and thickening. The patient underwent small bowel resection, which revealed scattered erosion with hemorrhage in the intestinal mucosa. The postoperative pathology report included hemorrhagic enteritis and intestinal adhesion with fistula formation (Figure 3). The patient experienced dark bloody or tarry stools 2 wk after surgery (approximately 200-800 g/d). Antihemorrhagic, antacid therapy and nutritional support were administrated. Eventually, the patient was discharged and made a full recovery.

Figure 1 Anesthesia was administrated to the patient who underwent amputation of the bilateral extremities. The surgical area was sterilized in preparation for the operation.

Figure 2 The damaged small intestine was adhered into a mass with a fistula. Thickness and hyperplasia were noted on the small intestinal wall after the part of damaged intestine was cleaned.

Figure 3 Large necrotic lesions along with macrophage infiltration were observed in the intestinal mucosa. Collagen fiber hyperplasia was also present on the submucosal membrane.

RESULTS

Two patients from this study died of respiratory failure on the first and second postoperative day (post-traumatic days 22 and 12, respectively). One patient survived. Our patients had the following common characteristics: (1) Multiple severe trauma events with no direct penetrating gastrointestinal injury; (2) Multiple surgeries with impaired renal function and intermittent hemodialysis treatment; (3) Progressive abdominal pain and tenderness, and peritoneal irritation was present on post-traumatic days 10-38; (4) Abdominal operations confirmed segment ulcer, necrosis of the small intestine, hyperplasia and stiffness of the intestinal wall; and (5) Pathological examinations suggested submucosal hemorrhage, necrosis, fibrosis and hyalinization of the vascular wall.

DISCUSSION

Diagnosis of intestinal necrosis: Stress-induced intestinal necrosis

How did we make the diagnosis of intestinal necrosis in these patients? We reviewed the relevant literature and found no related cases. However, cases of stress-induced intestinal injury were relatively common[9-11]. Lu et al[10] studied the stress response in rats under heat conditions. They found severe intestinal mucosal damage associated with changes in gene expression that were related to stress-induced immune regulation in the rat small intestine. Smith et al[11] studied the stress response to early weaning in porcine intestines and found that early weaning in pigs can induce stress and lead to impaired mucosal barrier function. The response to trauma, as a comprehensive stress reaction, can cause stress-induced damage to multiple organs in the body[12,13]. However, stress-induced intestinal necrosis has not been reported previously. There are many reports of gastrointestinal feeding tubes leading to partial necrosis of the small intestine, which were thought to be the result of bacterial infections[14,15].

In the 3 patients in our study, no gastrointestinal feeding tubes were placed. After careful consideration, we made the diagnosis of “stress-induced intestine necrosis”. The differential diagnosis of “acute intestinal necrosis” includes the following: (1) “Stress-induced intestine necrosis” occurs in severe trauma or multiple surgeries, while “acute intestinal necrosis” occurs for unknown reasons and may be related to contaminated food[16]; (2) “Stress-induced intestinal necrosis” has a relatively slow onset, as the 3 patients in this study had signs of peritoneal irritation on post-trauma days 10-38; “acute intestinal necrosis” develops early, usually within 5 d; (3) “Stress-induced intestinal necrosis” has no obvious abdominal pain and mainly presents as abdominal distention with no signs of peritoneal irritation, while “acute intestinal necrosis” presents with acute abdominal pain and unbearable and obvious signs of peritoneal irritation; (4) With regard to intraoperative findings, “stress-induced intestinal necrosis” usually has intestinal epithelial hyperplasia, segmental ulceration induced necrosis and fistula formation, while “acute intestinal necrosis” has segmental necrosis without intestinal wall thickening; and (5) With regard to pathological results, “stress-induced intestinal necrosis” has submucosal necrosis, hyperplasia, fibrosis and a large number of macrophages and cells with hemosiderin within the tissue, while “acute intestinal necrosis” has fibrin deposition within the small arteries, intestinal hemorrhage and necrosis[17].

Reasons for intestinal necrosis after trauma

We believe that intestinal necrosis is associated with a continuous high level of stress due to primary turuma and traumatic complications. Under stress: (1) Intestinal mucosal permeability increases, which results in increases in peritoneal inflammatory exudate[18]; (2) Intestinal bacteria can translocate peritoneally and worsen peritoneal infections[19-21]; (3) The intracellular space within the endothelium of mesenteric vessels increases, allowing blood cells and plasma to leak through the connective tissue and vessel lamina, leading to vascular hyalinization, sclerosis and fibrosis; (4) As the response to stress continues, the sympathetic adrenergic medullary system is highly excited, which can lead to intestinal vasoconstriction and a reduction in the blood supply[22]; (5) As arteriosclerosis and contraction of the mesenteric vessels continues, the blood supply continues to decrease, resulting in hypoxia of the mucosal tissue, acidosis, mucosal necrosis and sloughing, and ulcers; and (6) Another explanation is that stress induces vagal inactivation and consequently suppresses the “cholinergic anti-inflammatory pathway” which might lead to intestinal injury ,even intestinal necrosis[23].

The 3 patients in this study suffered from long periods of crush injuries followed by several surgeries and intermittent hemodialysis. Their bodies were in a state of continuous stress. The long-term reduction in the mesenteric blood supply led to intestinal necrosis.

Treatment of stress-induced intestinal necrosis

Relative to acute intestinal necrosis, stress-induced intestinal necrosis is a slow progressive process that is difficult to identify. In the Wenchuan earthquake, we only made diagnoses of stress-induced intestinal necrosis for 3 cases. Two of these patients died, and 1 survived, indicating the difficulty of treating this condition. Due to the small number of cases analyzed, our treatment experience is limited, but the following recommendations can be made. First, the primary trauma should be controlled as soon as possible. Stress was continuously present in part because the primary injury was not treated directly. In the representative case, after both legs were amputated, the infection of the amputated surface was not well controlled, resulting in a second right leg amputation. In addition, intermittent dialysis after renal failure could cause intestinal ischemia-reperfusion injury. Second, early administration of appropriate vasodilator could improve intestinal microcirculation. Third, vasoconstrictors should be used as little as possible. As the blood pressure drops, the application of a vasoconstrictor can increase the blood supply of vital organs such as the heart and brain, but it can reduce the intestinal blood supply. Fourth, intestinal flora should be adjusted appropriately. Many studies indicate that in cases of trauma or weakened immune systems, the intestinal flora can translocate, leading to infections in other parts of the body. A large amount of inflammatory secretions were found in the abdominal cavity of all 3 patients in this study, which may be directly related to intestinal flora that translocated peritoneally. Fifth, the timing of surgery is a key factor. All 3 patients in this study underwent laparotomies after they showed signs of peritoneal irritation. As the results showed, the optimal timing might be prior to this point. We believe that the late diagnosis of intestinal necrosis occurred for the following reasons: (1) Stress-induced intestinal necrosis is a relatively slow process; (2) Intestinal ischemia will inevitably lead to inflammatory exudate. At the same time, the omentum and surrounding intestine can wrap around the injured intestinal regions; and (3) It is only after multiple necroses occur and inflammatory substances cannot be contained that the peritoneum will be stimulated, and signs of peritoneal irritation will be presented. Therefore, the optimal timing of surgery is before the signs of peritoneal irritation. Frequent abdominal ultrasounds and abdominal biopsies can help in determining the optimal timing of surgery.

Early signs/warnings/precautions

During intestinal stress-induced injury, fatty acid binding protein (FABP) is widely recognized as a specific marker of intestinal damage[24,25]. FABP is released by epithelial cells of the intestinal mucosa into the circulation following mucosal damage. Studies have shown that the plasma concentration of FABP gradually increases with the severity of shock. Pathological examinations have suggested that the intestinal mucosal damage was becoming progressively worse[26].

Therefore, we could use FABP as a routine test marker for patients with severe trauma. The increased plasma levels of FABP, combined with other abdominal physical signs and ultrasound results, could aid in early abdominal exploration and facilitate the early diagnosis of this condition.

COMMENTS

Background

Stress-induced intestinal injury is generally associated with damage to the intestinal mucosal barrier. This type of injury has been confirmed in many animal experiments, but few studies have reported stress-induced intestinal necrosis.

Research frontiers

There are many reports of partial of the small intestinal necrosis, which were thought to be the results of mesenteric thrombosis or bacterial infection. However, few authors have reported stress-induced intestinal necrosis.

Innovations and breakthroughs

In this paper, the authors investigated the possible reasons and suggested therapeutic plan of stress-induced intestinal necrosis resulting from the severe trauma of an earthquake. Conclound: Stress-induced intestinal necrosis is strongly associated with high level of stress from trauma and post-traumatic complications; For the therapeutic strategy, the primary trauma should be controlled as soon as possible, and timely exploratory laparotomy maybe an effective method for preventing and treating stress-induced intestinal necrosis.

Applications

The patients suffered stress-induced intestinal necrosis should be rarely identified. However, this disease is very dangerous, and even result in death. This paper will offer an alert and instructions for preventing and treating stress-induced intestinal necrosis due to trauma.

Terminology

Fatty acid binding protein is widely recognized as a specific marker of intestinal damage, and which is released by epithelial cells of the intestinal mucosa into the circulation following mucosal damage.

Peer review

In this paper, the authors reported 3 patients who suffered Wenchuan earthquake, had no direct abdominal trauma, and presented small intestinal necrosis diagnosed as “stress-induced intestinal necrosis”. This is an interesting paper since, effectively, few data have been published in this domain. And this paper may offer an alert and instructions for preventing and treating stress-induced intestinal necrosis due to trauma.