Jiang DK, Yao L, Wang WZ, Peng B, Ren WH, Yang XM, Yu L. TP53 Arg72Pro polymorphism is associated with esophageal cancer risk: A meta-analysis. World J Gastroenterol 2011; 17(9): 1227-1233 [PMID: 21448430 DOI: 10.3748/wjg.v17.i9.1227]
Corresponding Author of This Article
Long Yu, MD, PhD, The State Key Laboratory of Genetic Engineering, Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai 200433, China. longyu@fudan.edu.cn
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
De-Ke Jiang, Lei Yao, Wen-Zhang Wang, Bo Peng, Wei-Hua Ren, Xian-Mei Yang, The State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China
Long Yu, The State Key Laboratory of Genetic Engineering, Institute of Biomedical Science, Fudan University, Shanghai 200433, China
ORCID number: $[AuthorORCIDs]
Author contributions: Yu L designed the study; Jiang DK and Yao L performed the majority of the study and wrote the manuscript; Wang WZ, Peng B, Ren WH and Yang XM edited the manuscript.
Supported by the National 973 Program of China (No. 2004CB518605), the National 863 Project of China (No.2006AA020501), the National Key Sci-Tech Special Project of China (No.2008ZX10002-020), the Project of the Shanghai Municipal Science and Technology Commission (03dz14086) and the National Natural Science Foundation of China (No.30024001and 30771188)
Correspondence to: Long Yu, MD, PhD, The State Key Laboratory of Genetic Engineering, Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai 200433, China. longyu@fudan.edu.cn
Telephone: +86-21-65643954 Fax: +86-21-65643250
Received: May 14, 2010 Revised: August 17, 2010 Accepted: August 24, 2010 Published online: March 7, 2011
Abstract
AIM: To investigate the association between TP53 Arg72Pro polymorphism and esophageal cancer (EC) risk using meta-analysis.
METHODS: All eligible studies published before March 1, 2010 were selected by searching PubMed using keywords “p53” or “TP53”, “polymorphism” or “variation”, “esophageal” and “cancer” or “carcinoma”. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were assessed for EC risk associated with TP53 Arg72Pro polymorphism using fixed- and random-effects models.
RESULTS: Nine case-control studies involving 5545 subjects were included in this meta-analysis. Significantly reduced risk of EC was associated with TP53 genotypes for Arg/Arg + Arg/Pro vs Pro/Pro (OR = 0.73, 95% CI: 0.57-0.94, P = 0.014). Subgroup analyses according to the source of controls and the specimens used for determining TP53 Arg72Pro genotypes or sample size showed that significantly reduced risk was observed only in studies which have population-based controls (Arg/Arg vs Pro/Pro: OR = 0.56, 95% CI: 0.47-0.66, P < 0.001), and use white blood cells or normal tissue to assess TP53 genotypes of cases (Arg/Arg vs Pro/Pro: OR = 0.56, 95% CI: 0.47-0.65, P < 0.001) or include at least 200 subjects (Arg/Arg vs Pro/Pro: OR = 0.56, 95% CI: 0.47-0.65, P < 0.001). Analysis restricted to well-designed studies also supported the significantly decreased risk of EC (Arg/Arg vs Pro/Pro: OR = 0.54, 95% CI: 0.46-0.64, P < 0.001).
CONCLUSION: TP53 Arg72 carriers are significantly associated with decreased EC risk. Nevertheless, more well-designed studies are needed to confirm our findings.
Citation: Jiang DK, Yao L, Wang WZ, Peng B, Ren WH, Yang XM, Yu L. TP53 Arg72Pro polymorphism is associated with esophageal cancer risk: A meta-analysis. World J Gastroenterol 2011; 17(9): 1227-1233
Esophageal cancer (EC) is the eighth most common cancer and sixth most deadly cancer worldwide. China and southern and eastern Africa are the relatively high risk areas[1,2]. There are two main forms of EC histologically: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EA). ESCC constitutes the majority (over 90%) of EC, but the incidence rates of EA have sharply increased in many Western countries recently[3-5]. The development of EC is a multifactorial process associated with a variety of risk factors. The two major risk factors of EC are tobacco smoking and alcohol drinking[6-8]. Inherited predisposition may also explain the high rates of EC[9].
TP53 is a major regulator of the cell response to stress and serves as a tumor suppressor by inducing cell cycle arrest or apoptosis[10]. Inactivation of the TP53 signaling pathway has been seen in most human cancers[11]. Previously, polymorphisms of TP53 have been reported to be the possible risk factors for some kinds of tumors[12]. The most common polymorphism of TP53 is at the 72nd amino acid residue, with an arginine (Arg) to proline (Pro) change because of a G→C transverse[13]. Differences in the biochemical or biological characteristics of these wild-type TP53 variants have been reported[14]. The Arg72 variant can better induce apoptosis than the Pro72 variant, indicating that the two polymorphic variants of TP53 are functionally distinct, which may influence the cancer risk or treatment[15].
A number of studies have reported the role of TP53 Arg72Pro polymorphism in cancers such as cervical cancer[16], lung cancer[17], breast cancer[18], and gastric cancer[19], but little is known about the association of TP53 polymorphism with EC. In recent years, several studies focused on the association between TP53 Arg72Pro polymorphism and EC susceptibility, with inconsistent results[20-29]. Hence, we performed a meta-analysis of all eligible studies to estimate the association between TP53 polymorphism and the risk of EC.
MATERIALS AND METHODS
Publication search
We searched the articles using the terms “p53” or “TP53”, “polymorphism” or “variation”, “esophageal” and “cancer” or “carcinoma” in Medline database utilizing the PubMed engine, and all eligible studies were published before March 1, 2010. We evaluated all associated publications to retrieve the most eligible literatures. Their reference lists were hand-searched to find other relevant publications. Articles were limited to English language papers.
Inclusion and exclusion criteria
The following inclusion criteria were used to select literatures for the meta-analysis: (1) published in peer-reviewed journals; (2) articles about TP53 Arg72Pro polymorphism and risk of EC; and (3) containing useful genotype frequencies. The exclusion criteria were: (1) none-case-control studies; (2) control population including malignant tumor patients; (3) the genotype frequencies of control group departing from Hardy-Weinberg equilibrium (HWE); and (4) duplicated publications.
Data extraction
Two investigators (Jiang and Yao) reviewed and extracted information from all eligible publications independently, according to the inclusion and exclusion criteria listed above. An agreement was reached by discussion between the two reviewers whenever there was a conflict. The following items were collected from each study: first author’s surname, year of publication, country of origin, ethnicity, source of controls, specimens used for assessment of TP53 Arg72Pro genotypes, total number of cases and controls as well as numbers of cases and controls with Arg/Arg, Arg/Pro and Pro/Pro genotypes, respectively.
Statistic analysis
Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the association between TP53 Arg72Pro polymorphism and EC risk. The pooled ORs were performed for homozygote comparison (Arg/Arg vs Pro/Pro), dominant model (Arg/Arg + Arg/Pro vs Pro/Pro), and recessive model (Arg/Arg vs Arg/Pro + Pro/Pro), respectively. Stratified analyses were performed based on the source of controls, the specimens used for determining TP53 Arg72Pro genotypes and sample size (cases and controls in total). A Chi-square-based Q-test was performed to check the heterogeneity[30]. If P≥ 0.1 was obtained in the heterogeneity test, ORs were pooled according to the fixed-effects model (the Mantel-Haenszel model)[31], otherwise the random-effects model (the DerSimonian and Laird model) was used[32]. One-way sensitivity analyses were performed to evaluate the stability of the meta-analysis results[33]. The potential publication bias was estimated using Egger’s linear regression test by visual inspection of the Funnel plot. P < 0.05 was considered statistically significant in publication bias[34]. If publication bias existed, the Duval and Tweedie non-parametric “trim and fill” method was used to adjust for it[35]. All statistical tests were performed with the software STATA version 10.0 (Stata Corporation, College station, TX).
RESULTS
Study characteristics
Eighteen studies were identified through literature search and selection based on the inclusion criteria. By the extraction of data, seven articles which are not case-control studies and one review article were excluded. Among the remaining 10 studies, one study[29] was excluded due to the genotype frequencies of controls deviated from HWE. In one study[24], two groups of controls (a high risk population and a low risk population) were used. However, the genotype frequencies of the low-risk population controls deviated from HWE. Therefore, only the high-risk population controls of this study were included in the final analysis.
The characteristics of nine eligible case-control studies are summarized in Table 1. The sample size of the 9 studies ranged from 89 to 2178. In total, 2114 EC cases and 3431 controls were included in the meta-analysis. Distribution of TP53 genotype frequencies among EC cases and controls of the nine studies are shown in Table 2. The frequencies of heterozygote genotype among the cases of the studies using the specimens of exfoliated esophageal cells[21] or tumor tissues[23] were obviously lower than those of other studies. In studies with at least 200 samples, there was not a wide variation of Arg72 and Pro72 allele frequencies among controls, with the Arg72 allele frequencies ranging from 53% to 60%[20,22,24,26-28]. But in studies with less than 200 samples, the control groups represented diverse frequencies of the Arg72 allele, which were 37%[21], 48%[23] and 59%[25], respectively.
Table 1 Main characteristics of included studies in the meta-analysis.
When all the eligible studies were pooled into the meta-analysis, evidence was found in an association between significantly decreased EC risk and the variant genotypes of TP53 in the dominant model (OR = 0.73, 95% CI: 0.57-0.94, P = 0.014, Table 3). However, significant inter-study heterogeneity existed in all genetic models (Table 3). In order to figure out the main reasons of the heterogeneity among studies and obtain exact consequence on the relationship between TP53 Arg72Pro polymorphism and EC susceptibility, stratified analyses were then performed.
Table 3 Results of meta-analysis for TP53 Arg72Pro polymorphism and esophageal cancer risk.
In stratified analysis according to the source of controls, significant association between reduced EC risk and TP53 genotypes was found solely in subgroup of studies with population-based controls in all genetic models (homozygote comparison: OR = 0.56, 95% CI: 0.47-0.66, P < 0.001; dominant model: OR = 0.57, 95% CI: 0.50-0.66, P < 0.001; recessive model: OR = 0.80, 95% CI: 0.70-0.92, P = 0.001; Table 3). Significantly increased EC risk, however, was observed in the subgroup of a study with different source of controls selected from population and blood donors in homozygote comparison (OR = 2.33, 95% CI: 1.03-5.24, P = 0.041) and recessive model (OR = 2.71, 95% CI: 1.42-5.02, P = 0.003, Table 3). No evidence of association was observed in studies without clear presentation of hospital-based controls or the source of controls (Table 3).
We divided the included studies into four subgroups according to the specimens used. As a result, significantly reduced EC risk was found only in subgroups where white blood cells or normal tissue were used to determine TP53 genotypes in different genetic models (homozygote comparison: OR = 0.56, 95% CI: 0.47-0.65, P < 0.001; dominant model: OR = 0.58, 95% CI: 0.51-0.67, P < 0.001; recessive model: OR = 0.78, 95% CI: 0.68-0.88, P < 0.001; Table 3). Nevertheless, significantly excessive risk of EC was observed in the subgroup of a study using tumor tissue to extract genomic DNA for genotyping TP53 by homozygote comparison and recessive model (Table 3). This study also has different sources of controls. No significant association was observed in the studies using mixed specimens of white blood cells and tumor tissues or exfoliated esophageal cells to assess TP53 genotypes (Table 3).
We also stratified the included studies into two subgroups by sample size. One included studies with at least 200 participants, and the other included studies with less than 200 participants. Interestingly, studies in the former subgroup also used white blood cells or normal tissue as the specimens to assess TP53 genotypes, and significant association between reduced EC risk and TP53 genotypes was observed in all genetic models (Table 3). However, in the latter subgroup, significantly increased EC risk was found in homozygote comparison (OR = 2.21, 95% CI: 1.24-3.93, P = 0.007) and recessive model (OR = 1.73, 95% CI: 1.15-2.61, P = 0.009).
We performed the analysis only in well-designed studies with population-based controls with at least 200 participants using white blood cells or normal tissue to determine TP53 genotypes. Significantly decreased risk of EC was found in all genetic models (homozygote comparison: OR = 0.54, 95% CI: 0.46-0.64, P < 0.001; dominant model: OR = 0.56, 95% CI: 0.49-0.65, P < 0.001; recessive model: OR = 0.79, 95% CI: 0.69-0.91, P = 0.001; Figure 1).
Figure 1 Forest plots for the relationship between TP53 Arg72Pro polymorphism and esophageal cancer risk in studies with population-based controls with at least 200 participants, using white blood cells or normal tissues to determine TP53 genotypes.
A: Homozygote comparison; B: Dominant model; C: Recessive model. The first authors’ surname and year of publication are given in the left part of the figure. The size of the black square corresponding to each study is proportional to the sample size. The centre of each square represents the odds ratio (OR) and the horizontal line shows the corresponding 95% CI. The pooled OR was obtained using fixed-effects model and is represented by hollow diamond, where its centre indicates the OR and its ends correspond to the 95% CI. Arg: Arginine; Pro: Proline.
Sensitivity analysis
A single study involved in the meta-analysis was deleted each time to reflect the effects of individual data-set on the pooled ORs, and most of the corresponding pooled ORs were not materially altered (data not shown).
Publication bias
Both Begg’s funnel plot and Egger’s test were performed to assess the publication bias of literatures. Begg’s funnel plots did not reveal any evidence of obvious asymmetry except for heterozygote comparison and dominant model in the overall meta-analysis (figures not shown). The Egger’s test results suggested that publication bias was evident in heterozygote comparison (P = 0.003) and dominant model (P = 0.004), but not evident in homozygote comparison (P = 0.058) and recessive model (P = 0.389). The Duval and Tweedie non-parametric “trim and fill’ method was used to adjust for publication bias. Meta-analysis with and without using “trim and fill” method did not draw different conclusions (data not shown), indicating that our results were statistically robust.
DISCUSSION
Since the identification of TP53 Arg72Pro polymorphism[13], a number of studies[20-29] have investigated the genetic effect of this polymorphism on EC susceptibility, but the results are inconclusive. This led us to undertake the present meta-analysis, which could quantitify all the available data and might help us to distinguish the true from the false, to explore a more robust estimate of the effect of this polymorphism on EC risk. The main finding of our meta-analysis with 9 published studies including 2114 cases and 3431 controls is that TP53 Arg72 carriers are significantly associated with decreased EC risk, and the results of increased risk or no effect of this polymorphism on EC may be due to methodological errors such as selection bias, inappropriate specimens used for genotype assessment, or limited statistical power.
We found that the distribution of TP53 Arg72Pro genotypes in controls deviated from HWE in the study by Yang et al[29], although it has a relatively large sample size including 435 cases and 550 controls. Yang et al[29] reported that TP53 Arg/Arg genotype was associated with significantly increased EC risk (OR = 6.48, 95% CI: 4.65-9.03), which is contrary to our results of meta-analysis. It is well known that deviation from HWE may be due to genetic reasons including non-random mating, or the alleles reflecting recent mutations that have not reached equilibrium, as well as methodological reasons including biased selection of subjects from the population, or genotyping errors[36,37]. In despite of the reasons of disequilibrium, the results of genetic association studies might be spurious if the controls were not in HWE[38,39]. In order to guarantee the criteria for the eligible studies, only studies with controls in HWE were included in this meta-analysis.
In the present study, statistically significant inter-study heterogeneity of genotype effect was detected in different genetic models when all the eligible studies were pooled into the meta-analysis. Pooling despite the presence of heterogeneity may yield the mean of varying effect sizes, but the biological interpretation of such a mean and its clinical application would be very difficult[40,41]. Therefore, it is important to explore the source of heterogeneity rather than obtaining a potentially meaningless pooled summary measure[42]. In order to identify the source of heterogeneity and ascertain the exact genetic effect of TP53 Arg72Pro polymorphism on EC risk, we stratified the studies according to the source of controls, the specimens used for assessment of TP53 genotypes and sample size. We found that the heterogeneity was remarkably decreased when the studies were divided according to the specimens used and sample size, indicating that the two factors may contribute to the observed heterogeneity.
In two of the nine included studies where the specimens used for assessment of TP53 genotype were exfoliated esophageal cells[21] or tumor tissues[23], the frequencies of heterozygote genotype were obviously lower than those of other studies. This indicates that loss of heterozygosity (LOH) may exist and the distribution of TP53 genotypes in these cases may not be the same as that in normal tissue or cells. Generally, spurious results may be obtained from genetic association studies with inappropriate material for determining genotypes[16]. In our meta-analysis, significant association between TP53 Arg72Pro polymorphism and reduced EC risk was not observed in subgroups using inappropriate material to determine TP53 genotypes but only in the subgroups using white blood cells or normal tissues. Consequently, in genetic association studies, DNA from white blood cells or normal tissues should be used for determining genetic polymorphism, but not tumor tissue or exfoliated cells, in which LOH is a frequent event[43,44].
Lacking sufficient statistical power is an unnegligible problem in genetic association studies detecting the possible risk for the polymorphism[45]. It is likely that most genetic polymorphisms represent modest effects on disease susceptibility. An adequately powered study to detect single genetic associations would typically require a relatively large sample size, depending on the prevalence of the implicated polymorphism and the exact OR. Some of the eligible studies for our meta-analysis had a very small sample size and may have limited statistical power to detect a slight effect or may have generated a fluctuated risk estimate[46,47]. Carefully conducted meta-analysis of these data is essential to clarify whether these associations are true or not. Through stratified analysis, we found significantly increased EC risk associated with TP53 genotypes in subgroups of the studies with less than 200 participants, which was contrary to the results in subgroup of the studies with at least 200 participants. Given that all of the studies with a sample size of less than 200 used inappropriate specimens for determining TP53 genotypes, the results may be unreliable.
Some limitations of this meta-analysis should be addressed. Firstly, publication bias was detected for heterozygote comparison and dominant model in overall meta-analysis. The potential reason may be that results from small studies were more likely to be published if there was positive data reported. Therefore, well-designed studies with large sample size are required. Secondly, in the subgroup analyses by ethnicity, the included studies involved only Asians and Africans. Data concerning other ethnicities such as Caucasians were not found. For Africans, only one study was conducted, with a small sample size of 73 cases and 115 controls, which has not enough statistical power to find the real association. Thus, additional studies are warranted to evaluate the effect of this functional polymorphism on EC risk in different ethnicities, especially in Africans and Caucasians. Thirdly, lack of original data, including data of genotypes and environmental risk factors, of the included studies limited our further evaluation of potential gene-environment interaction, especially the interaction between human papillomavirus (HPV) infection and TP53 Arg72Pro polymorphism, which was investigated in several studies[23,48-50]. However, unlike HPV infection in cervical carcinoma, the role of HPV in the etiology of EC remains controversial[8]. A more precise analysis should be conducted if individual data are available.
Despite some limitations, the results of this meta-analysis still suggest that TP53 Arg72 allele is a protective factor for EC. The significantly reduced EC risk was found only in subgroup analyses of well-designed studies. Therefore, it is necessary to conduct large-sample studies using appropriate materials for assessment of genotypes, as well as homogeneous EC patients and unbiased selected controls. Such studies taking these factors into account may eventually lead to a better and comprehensive understanding of the association between TP53 Arg72pro polymorphism and EC risk.
COMMENTS
Background
Esophageal cancer (EC) is the eighth most common cancer and sixth most deadly cancer worldwide. A common polymorphism of TP53 at the 72nd amino acid residue, with an arginine (Arg) to proline (Pro) change because of a G→C transverse has been implicated as a risk factor for EC, but individual studies have been inconclusive or controversial.
Research frontiers
A number of studies have reported the role of TP53 Arg72Pro polymorphism in cancers such as cervical cancer, lung cancer, breast cancer, and gastric cancer, but the association of TP53 polymorphism with EC is not fully understood.
Innovations and breakthroughs
The present study demonstrated that TP53 Arg72 carriers are significantly associated with decreased EC risk, and suggested that increased risk or no effect of Arg72 variant on EC reported may be due to methodological errors such as selection bias, inappropriate specimens used for genotype assessment, or limited statistical power.
Applications
In this report, the association between TP53 Arg72Pro polymorphism and EC risk was observed, and the Arg72 allele decreased the EC risk, which is meaningful to early diagnosis, prevention and individual-based treatment of EC. Therefore, Arg72Pro polymorphism of the TP53 gene might be a potential therapeutic target for EC.
Terminology
TP53 is a major regulator of the cellular response to stress and serves as a tumor suppressor by inducing cell cycle arrest or apoptosis. Inactivation of the TP53 signaling pathway has been seen in most human cancers and polymorphisms of TP53 have also been reported to be the possible risk factors for some kinds of tumors.
Peer review
This study is an interesting meta-analysis on the association of TP53 ArgPro polymorphism with EC risk. Out of the 9 studies that survived the selection criteria, they found that TP53 Arg72 carriers were significantly associated with decreased EC risk. The authors concluded that previous reports of increased risk or no effect of this polymorphism on EC may be due to methodological errors such as selection bias, inappropriate specimen or limited statistical power and they give guidelines on how to avoid these pitfalls.
Footnotes
Peer reviewer: Yoshiharu Motoo, MD, PhD, FACP, FACG, Professor and Chairman, Department of Medical Oncology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293, Japan
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