Cloning and expression of ornithine decarboxylase gene from human colorectal carcinoma

Figure 1 RT-PCR product on 1% agarose gel. Lane 1: ODC RT-PCR product, 1480 bp in length; Lane 2: pQE30-ODC plas-mid digested by restriction enzyme BamH I and Sal I.

Figure 2 The pQE30-ODC expression vector including early promoter T5, Lac operator and 6His-Tag.

Figure 3 Expressed ODC protein on SDS-PAGE. Lane 1: M15 negative control; Lane 2-5: Expressed ODC proteins in M15 1 to 4 hour(s) after induction of IPTG; M: Protein marker; The arrow showed the expressed ODC protein.

Figure 4 Western blot shows a strip of 50 kD indicating the expression of ODC protein.