A mouse model of severe acute pancreatitis induced with caerulein and lipopolysaccharide

Figure 1 In the LPS group, the pancreas was slightly edemamous. ×100.

Figure 2 Microscopic section of the pancreas from the Cn group, showing the features of acute edematous pancreatitis notably interstitial edema. ×100.

Figure 3 In the Cn+LPS group, more diffuse focal areas of nonviable pancreatic parenchyma appeared obviously. ×100.

Figure 4 The histological change of the exo-pancreatic organs in the Cn+LPS group. A: Renal cells were swollen; ×100; B: The vacuoliza-tion of liver cells; ×400; C: A lot of erythrocyte and inflammatory cells also infiltrated in the cavity of pulmonary alveolus. ×100.

Figure 5 A few cytoplastic vacuoles formed in acinar cells in the LPS group. × 10000.

Figure 6 In the Cn group, a few cytoplastic vacuoles in acinar cell appeared, and ZGs were decreased in number. The RER and the mitochondria was slightly swollen. × 10000.

Figure 7 The ultrastructure change of the pancreatic acinus in the Cn+LPS group. A: Chromatin margination of nuclei was present, the swollen mitochondria appeared; × 7000; B: the number of vacuoles greatly increased and their volume also greatly increased; × 7000; C: the endoplasmic reticulum exhibited whorls; × 14000; D: The crista of mitochondria was decreased or disappeared. × 20000.

Figure 8 Comparison of NO concentration between groups(^-x±s) (n = 8). ^aP < 0.01, vs NS group; ^bP < 0.01, vs LPS group; ^cP < 0.05,vs Cn group.