Role of mitochondrial dysfunction in hydrogen peroxide-induced apoptosis of intestinal epithelial cells

Figure 1 Hydrogen peroxide induced apoptosis of SW-480 cells. 1: PBR322/Hinf I (75, 154, 220, 221, 298, 344, 396, 504, 517, 1632); 2, 3: cells from normal control; 4: 4 mmol/L H₂O₂-stimualted cells (1 h); 5: 4 mmol/L H₂O₂-stimualted cells (3h); 6: 400 μmol/L H₂O₂-stimualted cells (1 h) 7: 400 μmol/L H₂O₂-stimualted cells (3 h).

Figure 2 Hydrogen peroxide induced translocation of mitochondria. (A) cells from normal control; (B) 400 μmol/L H₂O₂-stimualted cells (1 h). (C) 400 μmol/L H₂O₂-stimualted cells (3 h); (D) 4 mmol/L H₂O₂-stimualted cells (1 h); (E) 4 mmol/L H₂O₂-stimualted cells (3 h).

Figure 3 a: Changes of mitochondrial membrane potential induced by hydrogen peroxide. (A) cells from normal control; (B) 400 μmol/L H₂O₂-stimualted cells (1 h); (C) 400 μmol/L H₂O₂-stimualted cells (3 h); (D) 4 mmol/L H₂O₂-stimualted cells (1h); (E) 4 mmol/L H₂O₂-stimualted cells (3 h). b: Effects of hydrogen peroxide on mitochondrial mem-brane potential of SW-480 cells (^aP < 0.05 vs control).

Figure 4 Cytochrome c release in SW-480 cells induced by hy-drogen peroxide (S-P×400). A: control cells, B: 400 μmol/L H₂O₂-treated cells (30 min), C: 4 mmol/L H₂O₂-treated cells (30 min).