Photodynamic inhibitory effects of three perylenequinones on human colorectal carcinoma cell line and primate embryonic stem cell line

Figure 1 Structures of the three perylenequinones for photodynamic activity.

Figure 2 Photodependent cytotoxic effects of EA on R366. 4 ES cells at various concentrations: (1) 10^-7 Mol/L, (2) 10^-6 Mol/L, (3) 10^-5 Mol/L, (4) 10^-4 Mol/L, (5) 10^-3 Mol/L respectively. Results are means ± SD of three independent experiments.

Figure 3 Dose-dependent relationship between EA doses and rate of Hce-8693 cell apoptosis. Cells were incubated for 2 h with 1 × 10^-6 Mol/L, 1 × 10^-5 Mol/L, 1 × 10^-4 Mol/L, 1 × 10^-3 Mol/L EA photosensitizer respectively and then irradiated. Results are means ± SD of three independent experiments.

Figure 4 Dose-dependent relationship between HA doses and rate of Hce-8693 cell apoptosis. Cells were incubated for 2 h with 1 × 10^-6 Mol/L, 1 × 10^-5 Mol/L, 1 × 10^-4 Mol/L, 1 × 10^-3 Mol/L HA photosensitizer respectively and then irradiated. Results are means ± SD of three independent experiments.

Figure 5 Dose-dependent relationship between HB doses and rate of Hce-8693 cell apoptosis. Cells were incubated for 2 h with 1 × 10-6 Mol/L, 1 × 10-5 Mol/L, 1 × 10-4 Mol/L, 1 × 10-3 Mol/L HB photosensitizer respectively and then irradiated. Results were means ± SD of three independent experiments.

Figure 6 (a)-(b) illustrated the different antiproliferative effects of photoactivated HA, EA and HB on human Hce-8693 cells.