Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells

Figure 1 Immunocytochemistry analysis of the effects of tachyplesin on the protein levels of mutant p53, p16, cyclin D1, CDK4 in SMMC-7721 cells ( × 536). The protein levels of mutant p53 (A), cyclin D1 (E), CDK4 (G) were high in SMMC-7721 cells while the levels of mutant p53 (B), cyclin D1 (F), CDK4 (H) were decreased by tachyplesin. The level of p16 protein was low in SMMC-7721 cells (C) while high in the tachyplesin-treated cells (D).

Figure 2 In situ hybridization analysis of p21^WAF1/CIP1 and c-myc mRNA in untreated and tachyplesin-treated SMMC-7721 cells ( × 536). The level of p21^WAF1/CIP1 mRNA was low in SMMC-7721 cells (A) but high in the tachyplesin-treated cells (B). High level of c-myc mRNA was observed in SMMC-7721 cells (C) while the level of c-myc mRNA in the tachyplesin-treated cells down-regulated (D).