Peptidergic innervation of human esophageal and cardiac carcinoma

Figure 1 GAL-immunostaining of esophageal carcinoma. Scat-tered nerve fibers (arrows) branched almost vertically from nerve bundles in connective tissue and entered into tumor parenchyma × 250.

Figure 2 L-ENK-immunoreactive nerve fibers in esophageal carcinoma. The fine nerve fiber (arrow) with varicosities on it encircled a tumor cell and contacted with it closely ×500.

Figure 3 GAL-immunostaining of esophageal carcinoma. Many nerve fibers entered into a nestlike cancerous tissue (T) convergently from collective tissue around it. The structure on the top right corner was amplified in Figure 4×125.

Figure 4 Higher magnification of the part on the top right in Fig. 3. GAL- immunoreactive nerve fibers entered into esoph-ageal carcinoma from peripheral areas × 250.

Figure 5 Section of Cardiac adenocarcinoma immunostained for GAL. Many immunoreactive nerve fibers with beads-like varicosities distributed in the parenchyma of tumor ×500.

Figure 6 GAL-immunoreactive nerve fibers extended into nestlike cancerous tissue formed by cardiac adenocarcinoma cells. The scattered nerve fibers contacted with tumor cells closely ×500.

Figure 7 Montage photographs showing the effects of esoph-ageal (a) and cardiac (b) carcinoma tissue block (T) on co-cul-tured DRG (G). On the side adjacent to tumor, DRG extending long and dense processes whereas on the opposite side, the pro-cesses are sparse and short (b), or no processes at all (a) ×125.