Expressing patterns of p16 and CDK4 correlated to prognosis in colorectal carcinoma

Figure 1 Expression patterns for p16 and CDK4 gene proteins. A, B: nuclear/cytoplasmic pattern of p16 protein in normal crypts (SP × 100, 200), C: overexpression pattern of p16 in the cytoplasm of colon cancer cells (SP × 400), D: overexpression pattern of CDK4 in the cytoplasm of colon cancer cells (SP × 400).

Figure 2 Results in p16 and CDK4 mRNA expressions as well as p16 gene methylation status on the promoter region. (A) and (B): mRNA levels of both p16 and CDK4 genes were el-evated in colorectal carcinoma (T), compared with their matched normal (N) tissues. (C) There was no methylation detected on p16 promoter region in all 24 cases of cancer tissue with residue adenoma. u: unmethylated, m: methylated, M: molecular marker (PBR322/Hae III).