Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma

Figure 1 Agarose gel analysis of PCR products for SSCP assay. Agarose gel separation was used to determine the specificity of PCR reaction. PCR products with only one specific band of right size in the gel can be used to SSCP assay. M: 100 bp ladder.

Figure 2 Some different electrophoresis patterns in SSCP assayof each exon. Arrows showed the differences, including extra bands, differential bands shifting, and so on.

Figure 3 The sequences of mutation site of 880 in two pairs of HCC tissues. (A) HCC sample 91K, 91L; (B) HCC sample C64K, C64L. ‘K’ represents HCC tissue and ‘L’ represents adjacent nontumorous liver tissue. Arrow indicated the site of 880.

Figure 4 The point mutation in position 880 has no effect on the telomerase inhibitory activity of LPTS-L. A. The ‘A’ in position 880 of LPTS-L transcript was mutated to ‘T’; B. The expression and purification of GST-LPTLm253-328, 1, without IPTG induction; 2, IPTG induction; 3, purified protein. C. The telomerase inhibiting activity assay in vitro of mutant protein LPTLm253-328 in SMMC-7721 cell extract. 1-4 were 0.1, 0.5, 1, 5 μg protein added in cell extract for 10 min, respectively.