Construction of IL-2 gene-modified human hepatocyte and its cultivation with microcarrier

Figure 1 Restriction enzyme analysis of pLNCX and pLNCIL-2

Figure 2 Morphological changes of cultured L-02/IL-2 and L-02 cells at 24 hours (× 100). A: L-02/IL-2 cells; B: L-02 cells

Figure 3 The growth curves of L-02/IL-2, L-02/Neo and L-02 cells

Figure 4 Integration of NeoR gene celL’s genomic DNA by PCR. 1: L-02/IL-2 cells; 2: L-02/Neo cells; 3: L-02 cells; 4: PCR DNA marker

Figure 5 Hepatocytes-anchored microcarriers linked in mass(× 200)

Figure 6 Hepatocyte with normal super-microstructures tightly anchors microcarrier (↑) (× 15000)