Synergistic effect of cell differential agent-II and arsenic trioxide on induction of cell cycle arrest and apoptosis in hepatoma cells

Figure 1 Immunofluorescence staining of Hoechst 3325872 h after 1. 0 µmol/L As₂O₃ + 1.0 g/L CDA-II administred in HepG2 cells (× 400). →: dispersive fluorescences in normal cells nuclei; △: compact particulate fluorescences in apoptosis cell nuclei

Figure 2 Comparison of different groups on numbers of BEL-7402 cell apoptosis. A. Main effect (As₂O₃): F = 0.387, sig. = 0.063, P > 0.05; B. Main effect (CDA-II): F = 0.670, sig. = 0.785, P > 0.05; C. Interaction (As₂O₃ + CDA-II): F = 22.450, sig. = 0.000, P < 0.05

Figure 3 DNA agarose gel electrophore I of hepatoma cell lines treated by As₂O₃ or CDA-II + As₂O₃ for 72 h. M: λ-DNA Marker VII; A (HepG2): controls; B (HepG2): 1.0 µmol·L^-1 As₂O₃ + 1.0 g·L^-1 CDA-II; C (BEL-7402): 1.0 µmol·L^-1 As₂O₃ + 1.0 g·L^-1 CDA-II; D (HepG2): 5.0 µmol·L^-1 As₂O₃

Figure 4 Comparison of DNA in various groups 4 days after medicinal treatment. P < 0.05 Compared with the controls

Figure 5 Flow cytometry 4 days after medicinal treatment. (A). 1.0 µmol/L As₂O₃; (B). 1.0 g/L CDA-II; (C). 1.0 µmol/L As₂O₃ + 1.0 g/L CDA-II; (D). Control.