Gastric Cancer
Copyright ©The Author(s) 2003.
World J Gastroenterol. Jan 15, 2003; 9(1): 59-64
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.59
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 Schematic diagram of angiostatin cDNA fragment.
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Identification of angiostatin cDNA insertion in pcDNA3. 1(+)-angio. M: λdsDNA/HindIII marker; 1: pcDNA3.1(+)-angio/HindIII; 2: pcDNA3.1(+)-angio; 3: pcDNA3.1(+)-angio/HindIII+XbaI; 4: pcDNa3.1(+)-angio/BamHI.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Cell clones transfected with pcDNA3. 1(+)-angio and pcDNA3.1(+) respectively. A: Cell clone pcDNA3.1(+)-angio transfected; B: Cell clone pcDNA3.1(+) transfected.
Figure 4
Open in New Tab Full Size Figure Download Figure
Figure 4 Angiostatin mRNA expression by Dot Blot analysis. 1: SGC7901; 2: SGC7901(+); 3:SGC7901-pcDNA3.1(+)-angio.
Figure 5
Open in New Tab Full Size Figure Download Figure
Figure 5 Angiostatin protein expression by Western Blot analysis. M: Marker; 1: SGC7901pcDNA3.1(+)-angio; 2: SGC7901pcDNA3.1(+)-angio; 3: SGC7901pcDNA3.1(+); 4: SGC7901.
Figure 6
Open in New Tab Full Size Figure Download Figure
Figure 6 Cell growth curves.
Figure 7
Open in New Tab Full Size Figure Download Figure
Figure 7 Cell cycle distributions by FACS analysis. A: SGC7901 transfected with pcDNA3.1(+)-angio; B: SGC7901.
Figure 8
Open in New Tab Full Size Figure Download Figure
Figure 8 Tumors formed in nude mice by SGC7901/pcDNA-angio, SGC7901/pcDNA, and SGC7901 cells (n = 5). A:SGC7901; B: SGC7901/pcDNA3.1(+); C: SGC7901/pcDNA3.1(+)-angio.
Figure 9
Open in New Tab Full Size Figure Download Figure
Figure 9 MVD in immunochemistry staining. 1: SGC7901/pcDNA-angio; 2: SGC7901/pcDNA; 3: SGC7901.