Silencing-specific methylation and single nucleotide polymorphism of hMLH1 promoter in gastric carcinomas

Figure 1 Detection of hMLH1 methylation by BstUI-COBRA assay. PCR products and methylated ssPCR products (294 bp) were digested into two small fragments (206 bp and 88 bp). Unmethylated ssPCR products were not digested by BstUI. T1, T2: primary gastric carcinomas; N1, N2: corresponding normal gastric mucosal samples; X2: xenograft of primary gastric carcinomas (T2) with hMLH1 methylation; NC1, NC2: negative control xenografts of primary gastric carcinomas without hMLH1 methylation; PC: PCR products of the hMLH1 templates not treated by bisulfite

Figure 2 DHPLC Chromatograms of the specific methylation of the hMLH1 promoter ssPCR products were analyzed at partial denaturing temperature 54 °C, point mutation mode. T1 and T2, primary gastric carcinomas; N1 and N2, the corresponding normal gastric mucosal samples; X2, the xenograft of T2 in nude mouse