Design of a ribozyme targeting human telomerase reverse transcriptase and cloning of it’s gene

Figure 1 Predicted second structure of the substrate RNA surrounding the selected cleavage site

Figure 2 Predicted bimolecular co-fold between the hammer-head ribozyme and the substrate RNA

Figure 3 Diagram of the cloning of the hammerhead ribozyme

Figure 4 Gel analysis of the plasmid containing the ribozyme gene. Lane 1: 200 bp DNA ladder; lane 2: λDNA/Hind III marker; lane 3: pGEMEX-1-RZ/Bgl II; lane 4: pGEMEX-1-RZ/Hind III; lane 5: pGEMEX-1-RZ/EcoR I; lane 6: pGEMEX-1-RZ/Not I; lane 7: pGEMEX-1-RZ

Figure 5 DNA sequencing of ribozyme gene