Effects of tachyplesin on proliferation and differentiation of human hepatocellular carcinoma SMMC-7721 cells

Figure 1 Effect of tachyplesin on the growth of SMMC-7721 cells.

Figure 2 Effect of tachyplesin on the mitotic index of SMMC-7721 cells.

Figure 3 Effects of tachyplesin on the morphology and ultra-structure of SMMC-7721 cells. A: SMMC-7721 cells ( × 536); B: SMMC-7721 cells treated with tachyplesin (× 536); C: The nucleo-cytoplasm ratio is large, the shape of nucleus is irregu-lar and cell organelles are not well-developed in the cytoplasm of SMMC-7721 cell (× 8800); D: The nucleo-cytoplasm ratio decreased, the shape of nucleus is regular and cell organelles are well-developed in the cytoplasm of tachyplesin-treated SMMC-7721 cell ( × 11000).

Figure 4 Effects of tachyplesin on the activies of γ-GT and TAT in SMMC-7721 cells.

Figure 5 Effects of tachyplesin on the levels of α-FP, PCNA, p21^WAF1/CIP1, c-myc proteins in SMMC-7721 cells ( × 536). A: The high level of α-FP in SMMC-7721 cells detected by immunocytochemistry; B: The level of α-FP in the tachyplesin-treated SMMC-7721 cells is decreased; C: The high level of PCNA in SMMC-7721 cells; D: The level of PCNA in the tachyplesin-treated SMMC-7721 cells is decreased; E: The low level of p21^WAF1/CIP1 in SMMC-7721 cells; F: The level of p21^WAF1/CIP1 in the tachyplesin-treated SMMC-7721 cells is increased; G: The high level of c-myc in SMMC-7721 cells; H: The level of c-myc in the tachyplesin-treated SMMC-7721 cells is decreased.