Over-expression of LPTS-L in hepatocellular carcinoma cell line SMMC-7721 induces crisis

Figure 1 Morphologic changes induced by LPTS-L in hepatoma cell line SMMC-7721. A. cells expressing control vector; B. large flat cells in crisis expressing LPTL.

Figure 2 The expression of GST fusion protein with LPTS-L. M: protein marker; 1. total protein before IPTG induction; 2. total protein after IPTG induction for 3 h; 3. purified GST fusion protein by affinity chromatography on glutathione-agarose column.

Figure 3 LPTS-L fusion protein inhibits telomerase activity in hepatocellular carcinoma cell line SMMC-7721 by TRAP assay in vitro. 1. SMMC-7721 cell extract alone; 2. plus 10 µg GST protein; 3-6. plus 0.1, 0.5, 1, 5 µg GST-LPTS-L fusion protein and the lane 7 is cell extract plus RNase.