Relationship between bilirubin free radical and formation of pigment gallstone

Figure 1 Simulation of ESR spectrum of bilirubin. (a) The experimental spectrum; (b): Simulating spectrum. (I) Superoxide radical; (II) Semiquinone radical; (III) Free electron of superoxideradical delocalized to tetrapyrrole (heterotropism is ignored).

Figure 2 ESR spectra of bilirubin treated with: (a) Bilirubin (from Sigma) as control; (b) ⁶⁰Co (100Gy); (c) ·OH (Fe (II) + EDTA); (d) O₂·^- (XO/XOD). The relative intensity of ESR peak is: a:b:c:d = 1:2.1:2.8:3.9

Figure 3 ESR spectra of spin-trapping of bilirubin free radical in solution. (a) DMPO-traping spectrum of bilirubin; (b) computer simulating spectrum; (c) Simulating spectru of DMPO-H; (d) Simulating spectrum of DMPO-OOH; (e) simulating spectrum of DMPO-CH₂R.

Figure 4 ^IH-NMR spectra of bilirubin. (A) commercial bilirubin (from Sigma); (B) bilirubin treated with ⁶⁰Co.

Figure 5 Leakage of lactate dehydrogenase (LDH) of hepa tocyte. 1. Control; 2-7. Treated with BR_VC, BR_comm + BSA, BR_comm, BR_Co, BR_Fe, BR_XO/XOD

Figure 6 Absorption curve of total and oxidezed glutathione level of hepatocyte. (A) Total glutathione; (B) Oxidized glutathione 1. Control; 2-7. Treated with BR_VC, BR_comm + BSA, BR_comm, BR_Co, BR_Fe, BR_XO/XOD

Figure 7 Scans of SDS-PAGE stained with Coomassie blue R-250. (A) 1. control, 2-4 human erythrocyte membrane treated with BR_Co, the irradation doses are 100, 50, 5 (Gy) respectively; (B) human erythrocyte membrane treated with BR_Fe, the concentrations of 1-3 are 16.67, 13.3, 6.7 (mmol/L) respectively

Figure 8 Fluorescence spectrum of erythocyte proteins labeled with fluorescamine. (A) In the precipitated proteins; (B) In the supernatants. 1. control, 2.BR_VC, 3.BR_comm, 4-5. BR_Feªª (10 and 20 mmol/L) 6. BR_Co (100 Gy)

Math 1 Math(A1).