Nitric oxide and calcium ions in apoptotic esophageal carcinoma cells induced by arsenite

Figure 1 Ultrastructure of SHEEC1 cell treated with 3 μmol·L^-1 As₂O₃. Apoptotic appearance displayed with swelling of mitochondria and nuclear chroma-tin coagulating and merginating. m, mitochondria. n, nucleus. EM, × 7000

Figure 2 DNA histogram of SHEEC1 cell in 24 h after 3 μmol·L^-1 of As₂O₃ adding. ap, apoptotic peak.

Figure 3 NO determination of SHEEC1 treated with different concentra-tion of As₂O₃. NO increased markedly in 5 μmol·L^-1 of As₂O₃ group (A), intermediately in 3 μmol·L^-1 of As₂O₃ group (B) and lowly in 1 μmol·L^-1 of As₂O₃ group (C). The control group, 0 μmol·L^-1 of As₂O₃, were remained on the basal lines (D).

Figure 4 Dynamic changes of intracellular calcium in 7 cells of SHEEC1 treated with As₂O₃. A, SHEEC1 cells treated with As₂O₃ in 3 μmol·L^-1. B, Control group without adding As₂O₃.