Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide

Figure 1 DNA histogram of SHEEC1 cells 24 h after Figure 2 Apoptotic changes 24 h after As₂O₃.

Figure 2 As₂O₃ adding. ap, apoptotic peak. adding, HE × 400.

Figure 3 Apoptotic cells (EM x 15000). Mitochondria in 1-2 arrays located around the cell nucleus, not adding As₂O₃; Increment of mitochondria 2-4 h after As₂O₃ adding; Dense substances deposition in mitochondria 4-6 h after As₂O₃ adding; Apoptotic cell showed cell nucleus shrank, chromatin agglutinated, mitochondria increased and swelled as balloon-like 24 h after As₂O₃ adding.

Figure 4 The histogram of mitochondrial fluorescent intensity by FCM after As₂O₃ adding. A: Control; B: 2-4 h; C: 4-6 h; D: 12-24 h.

Figure 5 Increment of mitochondria with Rho123 labeled in cytoplasm of SHEEC1 after 2-4 h As₂O₃ adding. × 1000