Effect of cholesterol liposomes on calcium mobilization in muscle cells from the rabbit sphincter of Oddi

Figure 1 Cultured SO cells characterized with á-SM actin positive staining, filament were demonstrated along the longitudinal axis of the cells.

Figure 2 At the presence of extracellular Ca²⁺, intracellular Ca²⁺ concentra-tion changed under the LSCM(1 image·s^-1) after the addition of agonists, and it shows spatially heterogeneous alteration of fluorescent intensity in SO cells.

Figure 3 Intracellular fluorescence increases induced by different agonists. Ca2+ Concentration increased from resting level of 108 ± 21 nmol·L^-1 to 297 ± 66 nmol·L^-1, 275 ± 58 nmol·L^-1 and 251 ± 45 nmol·L^-1, respectively. ANOVA was performed for the analysis, F = 0.9184, no significant difference be-tween three groups (n = 4).

Figure 4 Effect of different antagonists on ACh induced cellular Ca²⁺ increase (^-x ± S^-x, n = 4). Maximal Ca²⁺ concentration increased to 192 ± 22 nmol·L^-1,175 nmol·L^-1± 26 nmol·L^-1 and 186 ± 30 nmol·L^-1, respectively. ANOVA was performed for the analysis, F = 1.324, with no significant difference be-tween the three experimental groups (n = 4 ).

Figure 5 Effect of different antagonists on CCK induced cellular Ca²⁺ increase (^-x ± S^-x, n = 4). Maximal Ca²⁺concentration increased to 220 ± 26 nmol·L^-1, 133 ± 21 nmol·L^-1 and 201 ± 40 nmol·L^-1, respectively. SNK-q test was performed, there was a statistical difference between heparin treated group and other two groups, P < 0.01.

Figure 6 Effect of cholesterol liposome on cellular Ca²⁺ mobilization (^-x ± S^-x, n = 4). Agonists induced cellular influorescence increase percentages were markedly less than that of control group.