CCK8 inhibits expression of TNF-α in the spleen of endotoxic shock rats and signal transduction mechanism of p38 MAPK

Figure 1 Mean arterial pressure (MAP) of animals injected normal saline, LPS, CCK + LPS and CCK, n = 6. ^aP < 0.05, ^bP < 0.01, vs Control, ^cP < 0.05, ^dP < 0.01, vs LPS

Figure 2 Effects of CCK on TNF-α , IL-1β and IL-6 2 h(TNF-α) or 6 h (IL-1β and IL-6) following LPS administration. n = 6. ^aP < 0.05, ^bP < 0.01, vs Control; ^cP < 0.05, ^dP < 0.01, vs LPS.

Figure 3 Effect of CCK-8 on LPS-induced TNF-α mRNA expression. Total RNA from the rat spleen was extrated at 30 min(A) or 2h(B) after LPS administration. n = 3. ^aP < 0.01 vs Control; ^bP < 0.01 vs LPS.

Figure 4 CCK-8 increases p38 MAPK activationon induced by LPSin spleen. ^aP < 0.05, ^bP < 0.01 vs Control; ^cP < 0.01 vs LPS.