Molecular mechanism about lymphogenous metastasis of hepatocarcinoma cells in mice

Figure 1 Changes of growth fraction of lmphocytes (G₂ + S/G₁ + G₂ + S + M) in draining lymph nodes of Hca-F/Hca-P burden mice.

Figure 2 MMPs activity of Hca-F and Hca-P cells in RPMI 1640 with or without lymph node extract using zymographic analysis. 1. lymph node extract; 2. Type-four collagenase; 3. Hca-F cells; 4. Hca-P cells; 5. Hca-F cells in lymph node extract; 6. Hca-P cells in lymph node extract.

Figure 3 MMPs activity of Hca-F and Hca-P cells in liver extract by using zymographic analysis. 1. Type-four collagenase; 2. Hca-F cells in liver extract; 3. Hca-P cells in liver extract; 4. Liver extract.

Figure 4 MMPs activity of Hca-F and Hca-P cells in spleen extract by using zymographic analysis. 1. Type-four collagenase; 2. Hca-F cells in spleen extract; 3. Hca-P cells in spleen extract; 4. Spleen extract.

Figure 5 Fas-L expressed in Hca-F and Hca-P cells. A: Fas-L expressed in Hca-F cells; B: in Hca-P cells; C: in metastatic Hca-F cells.

Figure 6 TUNEL of metastatic lymph node of Hca-F cells.