Preparation of single chain variable fragment of MG7 mAb by phage display technology

doi:10.3748/wjg.v7.i4.510

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Aug 15, 2001 (publication date) through Dec 21, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 15, 2001; 7(4): 510-514
Published online Aug 15, 2001. doi: 10.3748/wjg.v7.i4.510

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Figure 1 RT-PCR of V_H, V_L and ScFv fragment of MG₇ antibody. 1: V_H; 2: V_L; 3: ScFv; M: 100 bp ladder

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Figure 2 Enzymatic analysis of MG₇ recombinant phage antibody library with Eco RI and Hin dIII. 1-8: Recombinant clones from library; M: λ/Eco RI and Hin dIII

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Figure 3 Measurement of the relative molecular weight of soluble MG₇ ScFv. 1-3: Periplasmic extracts; 4: Neg. ctrl; M: Low molecular mass protein marker

Citation: Yu ZC, Ding J, Nie YZ, Fan DM, Zhang XY. Preparation of single chain variable fragment of MG₇ mAb by phage display technology. World J Gastroenterol 2001; 7(4): 510-514
URL: https://www.wjgnet.com/1007-9327/full/v7/i4/510.htm
DOI: https://dx.doi.org/10.3748/wjg.v7.i4.510