Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma

doi:10.3748/wjg.v7.i4.500

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Aug 15, 2001 (publication date) through May 4, 2024

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Times Cited (40)

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 15, 2001; 7(4): 500-505
Published online Aug 15, 2001. doi: 10.3748/wjg.v7.i4.500

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Figure 1 Immunohistochemical staining for VEGF in adenocarcinoma tissues of the stomach. The positive signal was found mainly on the surface and in the cytoplasm of gastric cancer cells. × 400

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Figure 2 Immunohistochemical staining for flk-1/KDR in undifferentiated cancer tissues of the stomach. The positive signal was found on the surface of endothelial cells. × 100

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Figure 3 Immunohistochemical staining for flk-1/KDR in adenocarcinoma tissues of the stomach. It was found mainly on the surface and in the cytoplasm of gastric cancer cells. × 200

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Figure 4 PCR analysis of SGC-s-hVEGF and SGC-as-hVEGF. a. SGC-s-hVEGF/SP₆+b; b. PCR marker; c. SGC-s-hVEGF/SP₆+a; d. SGC-as-hVEGF/SP₆b; e. SGC-as-hVEGF/SP₆+a

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Figure 5 Changes in cell surface VEGF protein as deter mined by flow cytometer immunofluorescence staining analysis. (A) Flow cytometric detection of VEGF changes of control. (B) Flow cytometric detection of VEGF changes of SGC-7901. (C) Flow cytometric detection of VEGF changes of SGC/s-hVEGF. (D) Flow cytometric detection of VEGF changes of SGC/as-hVEGF.

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Figure 6 The growth curve of different transfectant.

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Figure 7 The volume of tumors in nude mice (n = 5/group) s. c. developing from s.c. injection totaling 1.4 × 10⁶ cells/ animal of the different transfectants.

Citation: Liu DH, Zhang XY, Fan DM, Huang YX, Zhang JS, Huang WQ, Zhang YQ, Huang QS, Ma WY, Chai YB, Jin M. Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma. World J Gastroenterol 2001; 7(4): 500-505
URL: https://www.wjgnet.com/1007-9327/full/v7/i4/500.htm
DOI: https://dx.doi.org/10.3748/wjg.v7.i4.500