Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells

Figure 1 Analysis of recombinant plasmid by restriction endonuclease digestion. 1. λ DNA/Eco R I + Hind III; 2. PCR product; 3. pHTNS5/Bam H I + Kpn I; 4. pFHTNS5/Bam H I + Kpn I.

Figure 2 Construction of recombinant plasmid pFHTNS5.

Figure 3 Morphology of uninfected, transfected and infected Sf9 cells (100 ×). A: Uninfected cells; B: Transfected and infected cells

Figure 4 SDS-PAGE analysis of expressed HGV NS5 protein. 1. Uninfected sf9 cells; 2. sf9 cells infected with recombinant viruses; 3. Protein relative molecular mass standards. Arrow indicates the position of recombinant protein.

Figure 5 Western-blot analysis of recombinant protein HGV NS5. 1. Uninfected sf9 cells; 2. sf9 cells infected with recombinant viruses; 3. Protein relative molecular mass standards.