Radioimmunoimaging of colorectal cancer using 99mTc-labeled monoclonal antibody

Figure 1 Chromatogram of digested Hb3. OD₂₈₀ (·-·) shows concentration of protein. OD₄₀₅ (+ - +) shows immunoreactivity.

Figure 2 SDS-PAGE results of antibody electrophoresis. Lane 1: low molecular weight marker; lane 2-3: intact Hb3; lane 4-5: intact Hb3 (1/10 concentration); lane 6: Hb3 fragments F(ab’)₂ (broken); lane 7: Hb3 fragments F(ab’)₂ (unbroken); lane 8: high molecular weight marker; lane 9: low molecular weight marker.

Figure 3 Chromatogram of ^99mTc labeled antibody, OD₄₀₅ shows immunoreactivity. ·-· radioactivity, + - + immunoreactivity.

Figure 4 Images of Patient 4. Rectal cancer complicated with liver metastasis. Anterior scan (6 h) shows an unclear abnormal radiation concentration under the bladder (upper left). Anterior scan (24 h) shows a clear abnormal radiation concentration in the left lobe of liver (lower left).