Atrophic gastritis and gastric cancer tissue miRNome analysis reveals hsa-miR-129-1 and hsa-miR-196a as potential early diagnostic biomarkers

Figure 1 Results of microRNA differential expression analysis. A: Differentially expressed gastric tissue microRNAs among different conditions. P-adjusted < 0.05 and |log2 fold change| > 1; B: Multidimensional scaling plot based on normalized data showing a clustering corresponding to control, atrophic gastritis, gastric cancerous and adjacent tissues. The density plots show distributions of the first and second dimensions. CON: Control; AG: Atrophic gastritis; GC: Gastric cancerous; GCaj: Gastric adjacent tissue; MDS: Multidimensional scaling.

Figure 2 Venn diagram representing the number of commonly and uniquely differentially expressed microRNAs in three different comparison groups. P-adjusted < 0.05 and |log2 fold change| > 1. CON: Control; AG: Atrophic gastritis; GC: Gastric cancer.

Figure 3 Receiver operating characteristic curves showing prediction performances of expression levels. A-C: Hsa-miR-129-1-3p; D-F: Hsa-miR-196a-5p in tissue samples between different comparison groups: Control vs atrophic gastritis; control vs gastric cancer; and atrophic gastritis vs gastric cancer. AUC: Area under the curve; CON: Control; AG: Atrophic gastritis; GC: Gastric cancer.

Figure 4 Hsa-miR-129-1-3p and hsa-miR-196a-5p expression levels in study comparison groups. A: Atrophic gastritis and gastric cancer tissue samples compared to controls; B: Atrophic gastritis and gastric cancer plasma samples compared to controls. Box plot graphs; boxes correspond to the median value and interquartile range. CON: Control; AG: Atrophic gastritis; GC: Gastric cancerous; GCaj: Gastric adjacent tissue.