Liver infiltration of multiple immune cells during the process of acute liver injury and repair

Figure 1 Immune cell infiltration during the process of carbon tetrachloride-induced liver injury and repair. A-C: The levels of serum alanine transaminase, aspartate transaminase and total bilirubin in carbon tetrachloride (CCl₄)-treated wild-type mice; D-H: Quantitative changes in the infiltration of various immune cells in the livers of CCl₄-treated wild-type mice. The data represent the mean ± SD. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. ctrl: Control; ns: Not significant; ALT: Alanine transaminase; AST: Aspartate transaminase; TBil: Total bilirubin.

Figure 2 Immune cell infiltration during the process of concanavalin A-induced liver injury and repair. A-C: The levels of serum alanine transaminase, aspartate transaminase and total bilirubin in concanavalin A (ConA)-treated wild-type mice; D-H: Quantitative changes in the infiltration of various immune cells in the livers of ConA-treated wild-type mice. The data represent the mean ± SD. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. ctrl: Control; ns: Not significant; ALT: Alanine transaminase; AST: Aspartate transaminase; TBil: Total bilirubin.

Figure 3 The expression of leukocyte cell-derived chemotaxin 2 was increased in acute liver injury models. A: The relative expression of Lect2 mRNA in the liver tissues of carbon tetrachloride (CCl₄)-treated wild-type mice; B The levels of serum leukocyte cell-derived chemotaxin 2 (LECT2) in CCl₄-treated wild-type mice; C: The relative expression of Lect2 mRNA in the liver tissues of concanavalin A (ConA)-treated wild-type mice; D: The levels of serum LECT2 in ConA-treated wild-type mice. The data represent the mean ± SD. ctrl: Control; ns: Not significant; ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. ctrl: Control; ns: Not significant; LECT2: Leukocyte cell-derived chemotaxin 2.

Figure 4 Lect2-KO mice showed less severe liver injuries. A and B: The levels of serum alanine transaminase (ALT) and aspartate transaminase (AST) in the wild-type and Lect2-KO mice, with or without carbon tetrachloride treatment; C and D: The levels of serum ALT and AST in the wild-type and Lect2-KO mice, with or without concanavalin A treatment. The data represent the mean ± SD. ^aP < 0.05; ^bP < 0.01. ns: Not significant; ALT: Alanine transaminase; AST: Aspartate transaminase.

Figure 5 The effect of leukocyte cell-derived chemotaxin 2 on immune cell infiltration in the carbon tetrachloride-induced acute liver injury model. A-E: Quantitative changes in the infiltration of various immune cells in the livers of wild-type and Lect2-KO mice, with or without carbon tetrachloride (CCl₄) treatment; F: Representative images of infiltrated immune cells in CCl₄-treated wild-type and Lect2-KO mice, with or without CCl₄ treatment. The data represent the mean ± SD. ns: Not significant; ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. Scale bar: 50 μm.

Figure 6 The effect of leukocyte cell-derived chemotaxin 2 on immune cell infiltration in the concanavalin A-induced acute liver injury model. A-E: Quantitative changes in the infiltration of various immune cells in the livers of wild-type and Lect2-KO mice, with or without concanavalin A (ConA) treatment; F: Representative images of infiltrated immune cells in ConA-treated wild-type and Lect2-KO mice, with or without ConA treatment. The data represent the mean ± SD. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. ns: Not significant; Scale bar: 50 μm.

Figure 7 Chemokine-related genes changed in Lect2-KO mice. A: Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results for carbon tetrachloride (CCl₄)-treated Lect2-KO and wild-type mice; B: KEGG analysis results for concanavalin A (ConA)-treated Lect2-KO and wild-type mice; C: Heatmap of the differentially expressed chemokine-related genes between the Lect2-KO and wild-type mice after CCl₄ treatment; D: Heatmap of the differentially expressed chemokine-related genes between the Lect2-KO and wild-type mice after ConA treatment.

Figure 8 Leukocyte cell-derived chemotaxin 2 promoted THP-1 monocyte chemotaxis. A: Chemotactic effects of Lect2 on THP-1 cells when treated with different doses; B: Comparison of chemotactic effects between Lect2 and MCP-1. The data represent the mean ± SD. ^aP < 0.05; ^bP < 0.01. ctrl: Control.